EAS FISH for EBV EBER1 is Sensitive to Prior RNase treatment

Slides:

Advertisements

Similar presentations

Fluorescent In Situ Hybridization (FISH) to Identify Genetic Changes in Fine Needle Biopsy of Lung Lesions Prepared by Jin Jen NCI.

Advertisements

Long-Term Complete Regression of Nodal Marginal Zone Lymphoma Transformed Into Diffuse Large B-Cell Lymphoma With Highly Active Antiretroviral Therapy.

Immunohistochemistry and in situ hybridization allow researchers to pinpoint the expression of their protein and nucleic acid targets, respectively.

Fig. 1. EGFR content as determined by fluorescence in situ hybridization (FISH) and immunohistochemical staining. FISH was performed with the EGFR ( red.

Immune Profile and Epstein-Barr Virus Infection in Acute Interstitial Nephritis: An Immunohistochemical Study in 78 Patients Nephron Clin Pract 2011;119:c293–c300.

Fig. 1. In situ hybridization analysis for detecting endogenous mBest1 transcript in whole-brain region. In situ hybridization results from coronal (top.

Fig. 2. Histopathological findings for initial angioimmunoblastic T-cell lymphoma (AITL) of lymph node (A~C) and secondary diffuse large B-cell lymphoma.

Specificity of EAS FISH

Figure 2 In situ hybridization for rhinovirus type 14 (rhinovirus-14) in maxillary sinus biopsy specimens. A, Detection of rhinovirus-14, antisense probe,

Biological aspects of Epstein–Barr virus (EBV)-infected lymphocytes in chronic active EBV infection and associated malignancies Hirokazu Kanegane, Keiko.

Is Ureaplasma spp. the leading causative agent of acute chorioamnionitis in women with preterm birth? J. Kikhney, D. von Schöning, I. Steding, J. Schulze,

HS 4160 Critical Scientific Analysis

FISH Panel for Leukemic CTCL

Molecular Diagnosis of Epstein-Barr Virus-Related Diseases

The Frequency of Immunoglobulin Heavy Chain Gene and T-Cell Receptor γ-Chain Gene Rearrangements and Epstein-Barr Virus in ALK+ and ALK− Anaplastic Large.

تحليل الحساسية Sensitive Analysis.

Volume 15, Issue 8, Pages (May 2016)

Figure 4. Brain imaging and neuropathologic demonstration of Epstein-Barr virus (EBV) encephalitis in patient PT-10 Brain imaging and neuropathologic demonstration.

Is Ureaplasma spp. the leading causative agent of acute chorioamnionitis in women with preterm birth? J. Kikhney, D. von Schöning, I. Steding, J. Schulze,

Epstein–Barr Virus Infection Induces Aberrant TLR Activation Pathway and Fibroblast– Myofibroblast Conversion in Scleroderma Antonella Farina, Mara Cirone,

HVMNE, a novel lymphocryptovirus related to Epstein-Barr virus, induces lymphoma in New Zealand White rabbits by Maria G. Ferrari, Emilia D. Rivadeneira,

Li Cui, Raymond K. Blanchard, Robert J. Cousins Kidney International

Figure 3 Immunohistochemical analyses of positive and negative Epstein-Barr virus (EBV) control tissues using immunostaining Immunohistochemical analyses.

Rituximab Therapy for Epstein-Barr Virus–Related Chronic Hepatitis Following Living Donor Kidney Transplantation Kazuhide Ohta, MD, PhD, Masaki Shimizu,

Figure 3.Low power view revealed a bluish mass involving the intestinal wall (H-E, x40). Scattered benign histiocytes were present in the diffuse tumor.

Tait D. Shanafelt, MD, Timothy G. Call, MD Mayo Clinic Proceedings

Heat shock phenocopies E1B-55K late functions and selectively sensitizes refractory tumor cells to ONYX-015 oncolytic viral therapy Clodagh C. O’Shea,

Alicia K. Morgans, MD, Ran Reshef, MD, Donald E. Tsai, MD, PhD

Tumor Necrosis Factor Receptor-Associated Factor 1 Is Overexpressed in Reed-Sternberg Cells of Hodgkin’s Disease and Epstein-Barr Virus-Transformed Lymphoid.

The Development of a Multitarget, Multicolor Fluorescence in Situ Hybridization Assay for the Detection of Urothelial Carcinoma in Urine Irina A. Sokolova,

Use of EAS to define TCR-Vb3- and TCR-Cb1-expressing T cells

the common cytokine receptor g chain,

(A) WES of CSF-derived ctDNA collected prior to T-DM1 treatment.

Brenton T. Tan, Roger A. Warnke, Daniel A. Arber

Double probing individual human spermatozoa: aniline blue staining for persistent histones and fluorescence in situ hybridization for aneuploidies Laszlo.

Volume 56, Issue 5, Pages (November 1999)

Epstein-Barr virus in concomitant gastric carcinoma and adult T-cell leukemia/lymphoma Masanori Daibata, MD, Yuiko Nemoto, MD, Shigeki Takemoto, MD,

Blastic, angiocentric, Epstein-Barr virus positive B-cell infiltration from colon: haematoxylin and eosin stain (A), CD 20 immunostaining (B) and in situ.

Comprehensive Hybrid Capture–Based Next-Generation Sequencing Identifies a Double ALK Gene Fusion in a Patient Previously Identified to Be False-Negative.

Human Papillomavirus Gene Expression in Cutaneous Squamous Cell Carcinomas from Immunosuppressed and Immunocompetent Individuals Karin J. Purdie, Thirunavukarasu.

Volume 13, Issue 2, Pages (January 2003)

Focus on nasopharyngeal carcinoma

Volume 57, Issue 2, Pages (October 2000)

Presence of Epstein–Barr Virus in Langerhans Cells of CTCL Lesions

Volume 136, Issue 7, Pages (June 2009)

Expression and localization of histamine H2 receptor messenger RNA in human nasal mucosa Noriko Hirata, MDa, Kazuhiko Takeuchi, MDa, Kotaro Ukai, MDa,

Enterotypes of the distal gut microbial profiles.

Response to Crizotinib Observed in Lung Adenocarcinoma with MET Copy Number Gain but without a High-Level MET/CEP7 Ratio, MET Overexpression, or Exon.

D. Schmiedel, J. Kikhney, J. Masseck, P. D. Rojas Mencias, J

Volume 8, Issue 4, Pages (October 2001)

Yuri Oleynikov, Robert H. Singer Current Biology

Biallelic transcription of Igf2 and H19 in individual cells suggests a post-transcriptional contribution to genomic imprinting Y Jouvenot, F Poirier,

Intensity Transform Contrast Stretching Y ← u0+γ*(Y-u)/s

Ribosome Components Are Associated with Sites of Transcription

Figure Brain MRI findings before and during appearance of lymphoproliferative disorder and pathology findings of cerebellar lesion Brain MRI findings before.

Validation of Roche LightCycler Epstein-Barr Virus Quantification Reagents in a Clinical Laboratory Setting Margaret L. Gulley, Hongxin Fan, Sandra H.

Fig. 4. Detection of dFMR1 mRNA in dFMRP granules by FISH

G418 causes UPF1 to localize to cytoplasmic foci containing NMD substrates but not the P-body marker DCP1a. 6CFSMEo- cells were transfected with constructs.

In 6CFSMEo- cells, the NMD factor UPF1 colocalizes with NMD substrates under cytoskeleton disruptor treatment. 6CFSMEo- cells were transfected with constructs.

Karyotyping of human metaphase II oocytes by multifluor fluorescence in situ hybridization Julie M Clyde, Ph.D., Jan E Hogg, M.Sc., Anthony J Rutherford,

Expression of multiple forms of MEL1 gene products.

In situ hybridization. In situ hybridization. The expression pattern of VEGF-R2, angiopoietin 1 (Ang-1), angiopoietin 2 (Ang-2), the angiopoietin receptor.

Ribosomal RNA and β-catenin mRNA localize to newly formed presynaptic terminals. Ribosomal RNA and β-catenin mRNA localize to newly formed presynaptic.

Volume 155, Issue 5, Pages e117-e121 (May 2019)

Fig. 2. Comparison of endometrial glands in the decidua of EBV-infected (left) and EBV-uninfected (right) chorioamniotic membranes. EBER1 SISH (A), EBER1.

Reverse transcription (RT) in situ polymerase chain reaction (PCR) experiment using a single stranded biotinylated oligonucleotide probe for the detection.

A2/Melan-A multimer+ CD8+ allorestricted CTL clones derived from donor DR fail to significantly recognize DR self-targets. A2/Melan-A multimer+ CD8+ allorestricted.

by Honglin Chen, Paul Smith, Richard F. Ambinder, and S. Diane Hayward

Initial treatment of CLL: integrating biology and functional status

Expression of chemokine receptors in A-498 cell line.

Presentation transcript:

EAS FISH for EBV EBER1 is Sensitive to Prior RNase treatment JY(LCL), Epstein-Barr Virus transformed B cells, were probed with an antisense probe for EBER1 (filled histograms) or with a nonsense probe (open histograms). The probes were detected by fluorescence in situ hybridization (FISH) or by FISH with enzymatic amplification staining (EAS). The JY(LCL) cells shown in the right panel were treated with RNase prior to hybridization. These results demonstrate that EAS FISH is sensitive to prior RNase treatment. Standard FISH EAS FISH EAS FISH JY(LCL) JY(LCL) JY(LCL) prior treatment with RNase Log Fluorescence Intensity