EAS FISH for EBV EBER1 is Sensitive to Prior RNase treatment

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EAS FISH for EBV EBER1 is Sensitive to Prior RNase treatment JY(LCL), Epstein-Barr Virus transformed B cells, were probed with an antisense probe for EBER1 (filled histograms) or with a nonsense probe (open histograms). The probes were detected by fluorescence in situ hybridization (FISH) or by FISH with enzymatic amplification staining (EAS). The JY(LCL) cells shown in the right panel were treated with RNase prior to hybridization. These results demonstrate that EAS FISH is sensitive to prior RNase treatment. Standard FISH EAS FISH EAS FISH JY(LCL) JY(LCL) JY(LCL) prior treatment with RNase Log Fluorescence Intensity