Skin proliferation kinetics and in vivo fibroblast lineage tracing during dermal maturation (related to Fig 4)‏ Skin proliferation kinetics and in vivo.

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Skin proliferation kinetics and in vivo fibroblast lineage tracing during dermal maturation (related to Fig 4)‏ Skin proliferation kinetics and in vivo fibroblast lineage tracing during dermal maturation (related to Fig 4) A3D visualisation of the simulated mouse body segment. Colour code indicates epidermis in green, proliferating fibroblasts in blue and lumen in white.BQuantification of proliferating (Ki67‐positive) keratinocytes and fibroblasts in skin over time (n = 4 for 16.5, 29.5; n = 3 for 10.5, 18.5, 21.5, 30.5, 63.5, 69.5; n = 2 for 17.5, 19.5; biological replicates). Note that the decrease in proliferation of keratinocytes and fibroblasts follows similar kinetics over time.CRepresentative simulation images of the epidermal gradients at indicated MCS. Note that the signal concentrates in the immediate surroundings of the epidermal cells and decays over time.D, EIn vivo lineage tracing of upper (Blimp1+ and Lrig1+ cells) and lower dermis (Dlk1+ cells) fibroblasts. (D) Immunofluorescence image of tdtomato or CAG‐EGFP‐labelled fibroblasts (red) with the indicated Cre lines. Nuclei were labelled with DAPI (blue). (E) Quantification of the percentage of labelled fibroblasts in the lower dermis of adult mice (> P50) (n = 2 biological replicates). Note the increased abundance of Blimp1Cre‐ and Lrig1CreER‐labelled dermal fibroblasts in the lower dermal layer with age.Data information: Data shown are means ± s.d. Scale bar, 100 μm. Emanuel Rognoni et al. Mol Syst Biol 2018;14:e8174 © as stated in the article, figure or figure legend