Evaluation and Clinical Validation of Two Field–Deployable Reverse Transcription- Insulated Isothermal PCR Assays for the Detection of the Middle East.

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Presentation transcript:

Evaluation and Clinical Validation of Two Field–Deployable Reverse Transcription- Insulated Isothermal PCR Assays for the Detection of the Middle East Respiratory Syndrome–Coronavirus Yun Young Go, Yeon-Sook Kim, Shinhye Cheon, Sangwoo Nam, Keun Bon Ku, Meehyein Kim, Nam Hyuk Cho, Hyun Park, Pei-Yu Alison Lee, Yu-Chun Lin, Yun-Long Tsai, Hwa-Tang Thomas Wang, Udeni B.R. Balasuriya The Journal of Molecular Diagnostics Volume 19, Issue 6, Pages 817-827 (November 2017) DOI: 10.1016/j.jmoldx.2017.06.007 Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 POCKIT system workflow for point-of-need detection of Middle East respiratory syndrome–coronavirus (MERS-CoV) RNA. This system includes a compact automatic nucleic acid (NA) extraction device (taco mini) and a portable PCR device (POCKIT). After sample collection, nucleic acids were extracted using a preloaded extraction plate in approximately 30 minutes, and subsequently, the lyophilized reverse transcription-insulated isothermal PCR reaction was reconstituted and nucleic acids were added. The mixture was transferred to an R-tube and tested in a POCKIT device. TaqMan probe hydrolysis–based amplification signals were automatically detected, processed, and interpreted, providing qualitative results on the display screen in 60 minutes. Asterisk indicates sample collection method used in this study. The Journal of Molecular Diagnostics 2017 19, 817-827DOI: (10.1016/j.jmoldx.2017.06.007) Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions