Bile-Based Detection of Extrahepatic Cholangiocarcinoma with Quantitative DNA Methylation Markers and Its High Sensitivity So-Hyun Shin, Kyoungbun Lee,

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Bile-Based Detection of Extrahepatic Cholangiocarcinoma with Quantitative DNA Methylation Markers and Its High Sensitivity So-Hyun Shin, Kyoungbun Lee, Baek-Hui Kim, Nam-Yun Cho, Jin-Young Jang, Yong-Tae Kim, Donguk Kim, Ja June Jang, Gyeong Hoon Kang The Journal of Molecular Diagnostics Volume 14, Issue 3, Pages 256-263 (May 2012) DOI: 10.1016/j.jmoldx.2012.01.014 Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 A: Methylation levels of the DNA methylation markers that exhibited statistically significant differences in methylation levels between EHC and chronic cholecystitis tissue samples. B: Methylation frequencies of DNA methylation markers that showed statistically significant differences in methylation frequencies between EHC and chronic cholecystitis tissue samples. DNA methylation markers were sorted ordered from the highest to lowest P values from left to right along the x axis. Error bars indicate SEM. The Journal of Molecular Diagnostics 2012 14, 256-263DOI: (10.1016/j.jmoldx.2012.01.014) Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 Methylation maps of the PMR values obtained for 17 cancer-specific DNA methylation markers in a training set of bile fluid samples from patients with EHC (n = 20) and patients with cholecystitis or cholangitis (n = 20) (A) and a validation set of bile fluid samples from patients with EHC (n = 33) and patients with cholecystitis or cholangitis (n = 12) (B). Each circle in the cytology diagnosis column indicates a diagnosis of atypia or malignancy. Shading represents the methylation level (white, PMR < 1; light gray, 1 ≤ PMR < 20; dark gray, 20 ≤ PMR < 50; black, PMR ≥ 50). The Journal of Molecular Diagnostics 2012 14, 256-263DOI: (10.1016/j.jmoldx.2012.01.014) Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions