Right ventricular hypertrophy with early dysfunction: A proteomics study in a neonatal model  Amir M. Sheikh, MBBS, FRCS(C-Th), Cindy Barrett, MD, Nestor Villamizar, MD, Oscar Alzate, PhD, Anne Marie Valente, MD, J. Rene' Herlong, MD, Damian Craig, MS, Andrew Lodge, MD, Jeffrey Lawson, MD, PhD, Carmelo Milano, MD, James Jaggers, MD  The Journal of Thoracic and Cardiovascular Surgery  Volume 137, Issue 5, Pages 1146-1153 (May 2009) DOI: 10.1016/j.jtcvs.2008.09.013 Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Development of significant increase in RV mass was found by both MRI and on autopsy in the PAB animals. MRI demonstrated a significant decrease in RV ejection fraction (P < .05) and an increase in RV end-systolic volume (P = .06) in response to PAB of 4 weeks. The Journal of Thoracic and Cardiovascular Surgery 2009 137, 1146-1153DOI: (10.1016/j.jtcvs.2008.09.013) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Example of 2-dimensional differential gel electrophoresis of RV myocardium. The Cy2 (internal control) image is shown including identification numbers for the protein spots of interest. A typical 3-dimensional representation of a protein gel spot, as analyzed and displayed by DeCyder software, is shown below the gel, taking F1-ATPase β-chain (spot 1063) as an example. The Cy3 (sham) protein image is on the left and the Cy5 (PAB) image on the right. It can be seen there is a decrease of F1-ATPase β-chain peptide in the PAB animal. The Journal of Thoracic and Cardiovascular Surgery 2009 137, 1146-1153DOI: (10.1016/j.jtcvs.2008.09.013) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions