Detection of BRAF V600 Mutations in Metastatic Melanoma

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Detection of BRAF V600 Mutations in Metastatic Melanoma Kevin Qu, Qiulu Pan, Xi Zhang, Luis Rodriguez, Ke Zhang, Hairong Li, Albert Ho, Heather Sanders, Anthony Sferruzza, Shih-Min Cheng, Diedre Nguyen, Dan Jones, Frederic Waldman The Journal of Molecular Diagnostics Volume 15, Issue 6, Pages 790-795 (November 2013) DOI: 10.1016/j.jmoldx.2013.07.003 Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Sequencing amplification plots of BRAF codon 600. (A) V600 codon showing the wild-type GTG sequence. (B) V600E codon resulting from a single-nucleotide mutation (GTG>GAG). (C) V600E codon resulting from a dinucleotide mutation (GTG>GAA). (D) V600K codon resulting from a dinucleotide mutation (GTG>AAG). The top of each panel shows the sequence in the forward direction and the bottom portion shows the sequence in the reverse direction. The Journal of Molecular Diagnostics 2013 15, 790-795DOI: (10.1016/j.jmoldx.2013.07.003) Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions