Eutopic endometrial interleukin-18 system mRNA and protein expression at the level of endometrial-myometrial interface in adenomyosis patients Hong-Yuan.

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Eutopic endometrial interleukin-18 system mRNA and protein expression at the level of endometrial-myometrial interface in adenomyosis patients Hong-Yuan Huang, M.D., Hsing-Tse Yu, M.D., She-Hung Chan, B.Sc., Chyi-Long Lee, M.D., Hsin-Shih Wang, M.D., Ph.D., Yung-Kuei Soong, M.D., M.Phil. Fertility and Sterility Volume 94, Issue 1, Pages 33-39 (June 2010) DOI: 10.1016/j.fertnstert.2009.01.132 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Immunohistochemistry study of IL-18 system expression at the level of EMI in uterine adenomyosis. Immunoreactive IL-18 (A), IL-18 BP (B), and IL-18R (C) were identified in the human eutopic endometrium at the level of EMI using HRP staining. As a positive control, IL-18 was also studied in human placenta samples (D). A brown reaction product indicated a positive reaction for antibody staining. The inset photos were negative control of omitting the primary antibodies for matched tissues and targets (×200). Fertility and Sterility 2010 94, 33-39DOI: (10.1016/j.fertnstert.2009.01.132) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Quantitative amount of human endometrial IL-18 mRNA determined by QC-PCR in uterine adenomyosis and in control patients. Upper panel (A), the standard curve was obtained for IL-18 mRNA by plotting the logarithmically transformed ratios of the densities of target cDNA to competitive cDNA against the log amount of initially added target cDNA in each PCR. Lower panel (B), both IL-18 and IL-18BP mRNA was not significantly different in eutopic endometrium from adenomyosis patients in comparison to control group. Interleukin-18R mRNA was significantly increased in adenomyosis (∗P<.05). Values are mean ± SE. A, adenomyosis group (n = 19); C, control group (n = 9). Fertility and Sterility 2010 94, 33-39DOI: (10.1016/j.fertnstert.2009.01.132) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 3 The ratio of IL-18BP to IL-18 of human eutopic endometrium in uterine adenomyosis showed a significantly higher ratio at mRNA level in comparison to control group (P=.033). Values are mean ± SE. A, adenomyosis group (n = 19); C, control group (n = 9). Mann-Whitney-Wilcoxon analysis; ∗P<.05. Fertility and Sterility 2010 94, 33-39DOI: (10.1016/j.fertnstert.2009.01.132) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Pearson's correlation showed a significant correlation between endometrial IL-18 and IL-18R at the mRNA level in uterine adenomyosis (A) (P<.05), but not in control group (B). Fertility and Sterility 2010 94, 33-39DOI: (10.1016/j.fertnstert.2009.01.132) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions