H. Bretschneider, M. Stiehler, A. Hartmann, E. Boger, C. Osswald, J

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Presentation transcript:

Characterization of primary chondrocytes harvested from hips with femoroacetabular impingement H. Bretschneider, M. Stiehler, A. Hartmann, E. Boger, C. Osswald, J. Mollenhauer, C. Gaissmaier, K.-P. Günther Osteoarthritis and Cartilage Volume 24, Issue 9, Pages 1622-1628 (September 2016) DOI: 10.1016/j.joca.2016.04.011 Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Representative Safranin-O staining of two donors harvested from acetabular (left, A and C) and femoral (right, B and D) site; (A) acetabular site of donor 3 (Mankin-score 1–6); (B) femoral site (Mankin-score 8–12); (C) acetabular site of donor 5 (Mankin-score 9); (D) femoral site of donor 5 (Mankin-score 4). Osteoarthritis and Cartilage 2016 24, 1622-1628DOI: (10.1016/j.joca.2016.04.011) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Representative image of chondrocytes harvested from acetabular (left) and femoral (right) site after 19 days of monolayer culture (magnification 100×). Osteoarthritis and Cartilage 2016 24, 1622-1628DOI: (10.1016/j.joca.2016.04.011) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Gene expression of hip chondrocytes in different in vitro stages, 2D, monolayer cultivation, Sim, transport simulation; 3D, hydrogel-based cultivation, COL1, collagen 1; COL2, collagen 2; ACAN; IL-1β. Box plots indicate 25%/75% borders with the dots indicating outliers. The data were statistically analyzed by One-way ANOVA or Kruskal–Wallis One-way ANOVA on ranks. P-values <0.05 were considered significant (for details see Method section). Osteoarthritis and Cartilage 2016 24, 1622-1628DOI: (10.1016/j.joca.2016.04.011) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Expression of chondrogenic marker genes (COLI, COLII and ACAN) and IL-1β normalized to GAPDH after 2D and 3D cultivation from acetabular and femoral donor site; 2D, monolayer cultivation; 3D, hydrogel-based cultivation; COL1, collagen 1; COL2, collagen 2; ACAN; IL-1β; GAPDH. The data were statistically analyzed by Wilcoxon–Mann–Whitney test. P-values <0.05 were considered significant. Osteoarthritis and Cartilage 2016 24, 1622-1628DOI: (10.1016/j.joca.2016.04.011) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions