Induction of cell filamentation by Fic-1.

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Induction of cell filamentation by Fic-1. Induction of cell filamentation by Fic-1. (A) Cells from E. coli transformants expressing SUMO-tagged Fic-1 or its H135A mutant grown for 16 hours on LB agar resuspended in phosphate-buffered saline (PBS) were fixed and stained with Hoechst. (B) Cells of P. fluorescens grown in LB with glucose were diluted in ABM medium containing 0.2% arabinose and grown for 12 hours. Cells were treated for imaging as described in (A). Images acquired with a fluorescence microscope were pseudocolored with IPLab software. (C and D) Cells grown as described in (A) and (B) were processed for SDS-PAGE. Proteins were probed for Fic-1 by immunoblotting. The metabolic enzyme ICDH was probed as a loading control (lower panels). Similar results were obtained from at least three independent experiments. Canhua Lu et al., Sci. Signal. 2016;9:ra11 ©2016 by American Association for the Advancement of Science