R Kumar, M Mukherjee, M Bhandari, A Kumar, H Sidhu, R.D Mittal

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Role of Oxalobacter formigenes in Calcium Oxalate Stone Disease: A Study from North India R Kumar, M Mukherjee, M Bhandari, A Kumar, H Sidhu, R.D Mittal European Urology Volume 41, Issue 3, Pages 318-322 (March 2002) DOI: 10.1016/S0302-2838(02)00040-4

Fig. 1 Detection of O. formigenes using a genus-specific primer (Oxf-6/Oxf-7). Lanes 1–5, PCR products of normal individuals showing presence of the bacteria. Lane 6, 100bp DNA ladder. Lanes 7–9, PCR products of calcium oxalate stone formers showing absence of the bacteria. Lane 10, negative control. Lane 11, positive control. European Urology 2002 41, 318-322DOI: (10.1016/S0302-2838(02)00040-4)

Fig. 2 (a) PCR amplification of DNA from stool samples of calcium oxalate stone patients using Oxf-6/Oxf-7 primers. Lane M, 100bp DNA ladder. Lanes 1–4, absence of O. formigenes. Lanes 5 and 6, presence of O. formigenes. Lane 7, negative control. Lane 8, positive control. (b) Southern blot analysis of the above PCR products using genus-specific oligonucleotide γ32P-labelled probe Oxf-5. European Urology 2002 41, 318-322DOI: (10.1016/S0302-2838(02)00040-4)

Fig. 3 Bar diagram showing percentage colonization of O. formigenes in different groups of stone formers. Control, no stone episode; A, one stone episode; B, two stone episodes; C, three or greater than three stone episodes. European Urology 2002 41, 318-322DOI: (10.1016/S0302-2838(02)00040-4)

Fig. 4 PCR amplification of bacterial DNA in all stool samples used for the detection of O. formigenes using universal bacterial primers Bac-1/Bac-2. Lanes 1–13, show presence of bacterial DNA. Lane M, 100bp DNA ladder. European Urology 2002 41, 318-322DOI: (10.1016/S0302-2838(02)00040-4)

Fig. 5 Bar diagram showing the urinary oxalate levels in different groups of stone formers. Control, no stone episode; A, one stone episode; B, two stone episodes; C, three or greater than three stone episodes. European Urology 2002 41, 318-322DOI: (10.1016/S0302-2838(02)00040-4)