Phosphatase activity associated with Shp1 is reduced in Themis−/− thymocytes. Phosphatase activity associated with Shp1 is reduced in Themis−/− thymocytes.

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Phosphatase activity associated with Shp1 is reduced in Themis−/− thymocytes. Phosphatase activity associated with Shp1 is reduced in Themis−/− thymocytes. (A) Tyrosine phosphatase activity assay of immunoprecipitated Shp1 on ex vivo thymocytes from Themis+/+ and Themis−/− mice with and without stimulation using biotinylated antibody cross-linking with streptavidin. (B) Tyrosine phosphatase activity assay of immunoprecipitated Shp1 on sorted ex vivo preselection thymocytes from Themis+/+ and Themis−/− mice with stimulation using biotinylated antibody cross-linking with streptavidin. (C) Titration of Themis by comparing Shp1 activity in unstimulated and stimulated thymocytes using biotinylated antibody cross-linking with streptavidin from Themis+/+, Themis+/−, and Themis−/− mice. (D) Tyrosine phosphatase activity assay of immunoprecipitated Shp1 on ex vivo sorted peripheral T cells from Themis+/+ and Themis−/− mice with and without stimulation using biotinylated antibody cross-linking with streptavidin. For sorting, two Themis+/+ and four Themis−/− mice were used. Data are representative of three experiments. Unpaired t test with mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. N.S., not significant. Monika Mehta et al. PNAS 2018;115:48:E11331-E11340 ©2018 by National Academy of Sciences