Asymptomatic primary Epstein-Barr virus infection occurs in the absence of blood T-cell repertoire perturbations despite high levels of systemic viral.

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Asymptomatic primary Epstein-Barr virus infection occurs in the absence of blood T-cell repertoire perturbations despite high levels of systemic viral load by Sharon L. Silins, Martina A. Sherritt, Jodie M. Silleri, Simone M. Cross, Suzanne L. Elliott, Mandvi Bharadwaj, Thuy T. T. Le, Leanne E. Morrison, Rajiv Khanna, Denis J. Moss, Andreas Suhrbier, and Ihor S. Misko Blood Volume 98(13):3739-3744 December 15, 2001 ©2001 by American Society of Hematology

TCR Vβ repertoire analysis of PBMCs from IM donors during acute infection and recovery.Depicted are the Vβ values of the known 1 to 25 families with significant expansions. TCR Vβ repertoire analysis of PBMCs from IM donors during acute infection and recovery.Depicted are the Vβ values of the known 1 to 25 families with significant expansions. Significance was based on at least a 2-fold magnitude change and P < .05 against a control background (see “Materials and methods”). Profiles are displayed of fluorescence intensity (x-axis, arbitrary units) as a function of CDR3 size (y-axis, amino acids). CDR3 peaks are spaced 3 nucleotides apart. ND indicates not done; NP, not present. Sharon L. Silins et al. Blood 2001;98:3739-3744 ©2001 by American Society of Hematology

TCR clonotype sequences from acute IM donors TCR clonotype sequences from acute IM donors.TCR CDR3 region sequences of selected Vβ expansions that were found by direct sequencing and recombinant cloning to be monoclonal (see “Materials and methods”). TCR clonotype sequences from acute IM donors.TCR CDR3 region sequences of selected Vβ expansions that were found by direct sequencing and recombinant cloning to be monoclonal (see “Materials and methods”). TCR Vβ and Jβ gene elements were assigned according to Arden et al52 and Toyonaga et al,53 respectively. FW signifies framework branches that putatively support the CDR3 region. Nucleotide sequences of each these clones are available from EMBL/GenBank/DDBJ under accession numbersAJ308532 to AJ308537. Sharon L. Silins et al. Blood 2001;98:3739-3744 ©2001 by American Society of Hematology

TCR Vβ repertoire analysis of PBMCs from asymptomatic donors during acute and persistent infection.Depicted are those Vβ values of the 1 to 25 known families who demonstrated a 2-fold magnitude change (see “Materials and methods”). TCR Vβ repertoire analysis of PBMCs from asymptomatic donors during acute and persistent infection.Depicted are those Vβ values of the 1 to 25 known families who demonstrated a 2-fold magnitude change (see “Materials and methods”). Expansions that were statistically significant (P < .05) are indicated by an asterisk. Profiles are displayed of fluorescence intensity (x-axis, arbitrary units) as a function of CDR3 size (y-axis, amino acids). All CDR3 peaks are spaced 3 nucleotides apart. Sharon L. Silins et al. Blood 2001;98:3739-3744 ©2001 by American Society of Hematology

TCR clonotype sequences from acute asymptomatic donor AS4 TCR clonotype sequences from acute asymptomatic donor AS4.TCR CDR3 region sequences of selected Vβ expansions that were found by direct sequencing and recombinant cloning to be monoclonal (see “Materials and methods”). TCR clonotype sequences from acute asymptomatic donor AS4.TCR CDR3 region sequences of selected Vβ expansions that were found by direct sequencing and recombinant cloning to be monoclonal (see “Materials and methods”). TCR Vβ and Jβ gene elements were assigned according to Arden et al52 and Toyonaga et al,53 respectively. FW signifies framework branches that putatively support the CDR3 region. Nucleotide sequences of each of these clones are available from EMBL/GenBank/DDBJ under accession numbers AJ308538 to AJ308539. Sharon L. Silins et al. Blood 2001;98:3739-3744 ©2001 by American Society of Hematology

EBV load.In peripheral blood of IM patients (IM), asymptomatic seroconvertants (AS), healthy control donors (HC), and negative controls (water, EBV− PBMC, BJAB−). EBV load.In peripheral blood of IM patients (IM), asymptomatic seroconvertants (AS), healthy control donors (HC), and negative controls (water, EBV− PBMC, BJAB−). EBV genome copies were estimated by semiquantitative BMLF1 PCR-ELISA on PBMCs (see “Materials and methods”). UN indicates undetectable (less than 0). Specific descriptions of the IM and AS donors are outlined in Table 1. Sharon L. Silins et al. Blood 2001;98:3739-3744 ©2001 by American Society of Hematology