Telomere length in human blastocysts

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Presentation transcript:

Telomere length in human blastocysts Anastasia Mania, Anna Mantzouratou, Joy D.A. Delhanty, Gianluca Baio, Paul Serhal, Sioban B. Sengupta Reproductive BioMedicine Online Volume 28, Issue 5, Pages 624-637 (May 2014) DOI: 10.1016/j.rbmo.2013.12.010 Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Telomere quantitative FISH signals (red) with (right) and without (left) DAPI stain on embryonic cells. Bars=1μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Reproductive BioMedicine Online 2014 28, 624-637DOI: (10.1016/j.rbmo.2013.12.010) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Telomere quantitative FISH signals (red) with (right) and without (left) DAPI stain on lymphocyte cells. Bars=1μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Reproductive BioMedicine Online 2014 28, 624-637DOI: (10.1016/j.rbmo.2013.12.010) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Telomere quantitative FISH signals (red) with DAPI stain (blue) on keratinocyte cells. Bars=1μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Reproductive BioMedicine Online 2014 28, 624-637DOI: (10.1016/j.rbmo.2013.12.010) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

Figure 4 Variation in telomere intensity in parental lymphocytes and keratinocytes for each couple (in intensity units): (A) maternal lymphocytes, (B) paternal lymphocytes, (C) keratinocytes. For couples 1, 2, 3 and 4, only one keratinocyte control is shown because all embryo samples were ran in the same experiment; for couple 6, the telomere maternal control did not give consent to research and so is excluded. The maternal and paternal ages of couples 1–7 were 36 and 64years, 27 and 44years, 29 and 32years, 39 and 43years, 41 and 41years, 44 and 46years and 42 and 37years, respectively. Reproductive BioMedicine Online 2014 28, 624-637DOI: (10.1016/j.rbmo.2013.12.010) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

Figure 5 Variation in telomere length in individuals of different age groups, showing the sensitivity and accuracy of quantitative FISH: (A) 64-year-old paternal lymphocytes, (B) 41–46-year-old paternal lymphocytes. Reproductive BioMedicine Online 2014 28, 624-637DOI: (10.1016/j.rbmo.2013.12.010) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

Figure 6 Hierarchical regression coefficients. Values are mean and 95% confidence intervals. Intervals completely below (or above) zero indicate statistically significant negative (or positive) effects. Negative coefficients indicate a tendency to show shorter telomere in association with the variable represented by the coefficient. Blue=preimplantation genetic screening result or interaction term between cell- and embryo-level chromosomal abnormalities; red=embryo characteristics; yellow=embryo morphology (Blastocyst); green=couple characteristics; purple=paternal age. Baseline categories (e.g. minor mosaic for embryo characteristics) are represented by a single dot at zero and the coefficients of the other categories in the same group are incremental with respect to these. The interaction term was included to determine the existence of a differential effect of abnormalities between embryos that had reached the blastocyst stage and those that had not as shown in yellow (morulae and arrested embryos). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Reproductive BioMedicine Online 2014 28, 624-637DOI: (10.1016/j.rbmo.2013.12.010) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions