Remodeling of estrogen-dependent sympathetic nerve fibers seems to be disturbed in adenomyosis  Maria L. Barcena de Arellano, M.S., Jeannette Oldeweme, M.D., Julia Arnold, M.S., Achim Schneider, M.D., M.P.H., Sylvia Mechsner, M.D.  Fertility and Sterility  Volume 100, Issue 3, Pages 801-809.e2 (September 2013) DOI: 10.1016/j.fertnstert.2013.05.013 Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Formalin-fixed paraffin-embedded sections of the eutopic endometrium of women with and without AM stained against PGP 9.5 (A, B), against SP (C, D), and against TH (E, F) (original magnification ×200; arrows show NFs). (G) Quantification of the NF density of PGP 9.5, SP, and TH (NFs/mm2) in the eutopic endometrium of women without AM (mean density and SD; two-way ANOVA). (H) Quantification of the NF density of PGP 9.5, SP, and TH (NFs/mm2) in the eutopic endometrium of women with AM (mean density and SD; two-way ANOVA). Fertility and Sterility 2013 100, 801-809.e2DOI: (10.1016/j.fertnstert.2013.05.013) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Formalin-fixed paraffin-embedded sections of the myometrium of women with and without AM stained against PGP 9.5 (A, B), against SP (C, D), and against TH (E, F) (original magnification ×200; arrows show NFs). EM = endometrium; MM = myometrium; AL = AM lesion. (G) Quantification of the NF density of PGP 9.5, SP, and TH (NFs/mm2) in the myometrium of women with and without AM (mean density and SD; two-way ANOVA and Bonferroni post-test: ***P<.001). Fertility and Sterility 2013 100, 801-809.e2DOI: (10.1016/j.fertnstert.2013.05.013) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Formalin- fixed paraffin-embedded sections of the eutopic endometrium and myometrium of women with and without AM (A–F) stained against P450 aromatase and (G–K) double stained against ERα/TH or ERβ/TH. (A) Endometrium from women without AM, (B) endometrium from women with AM, (C) myometrium from women without AM, (D) myometrium from women with AM, and (E) AM lesion (original magnification ×200). (F) Quantification of the P450 aromatase expression in the eutopic endometrium and myometrium of women with and without AM and in adenomyotic lesion (mean density and SD; two way Anova with Bonferroni correction test; P<.05*). (G) ERα/TH staining of uteri without AM, (H) ERα/TH staining of uteri with AM, (I) ERβ/TH staining of uteri without AM, and (J) ERβ/TH staining from women with AM (original magnification ×200; arrows show NFs). (K) Quantification of the ERα/ERβ ratio in the myometrial noradrenergic NFs of women with and without AM (mean density and SD; two-way ANOVA). Fertility and Sterility 2013 100, 801-809.e2DOI: (10.1016/j.fertnstert.2013.05.013) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Formalin-fixed paraffin-embedded sections of adenomyotic implants (A, B, C) stained against PGP 9.5 (A), substance P (SP) (B), and TH (C) (original magnification ×200; arrows show NFs). (D) Quantification of the NF density of intact (PGP 9.5), peptidergic (SP), and noradrenergic (TH; NF/mm2) close to the adenomyotic implants (mean density and SD; Kruskal-Wallis test). Fertility and Sterility 2013 100, 801-809.e2DOI: (10.1016/j.fertnstert.2013.05.013) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions