Matthew C. Tunis, BSc, Wojciech Dawicki, PhD, Kaitlyn R

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Presentation transcript:

Mast cells and IgE activation do not alter the development of oral tolerance in a murine model Matthew C. Tunis, BSc, Wojciech Dawicki, PhD, Kaitlyn R. Carson, BSc, Jun Wang, PhD, Jean S. Marshall, PhD Journal of Allergy and Clinical Immunology Volume 130, Issue 3, Pages 705-715.e1 (September 2012) DOI: 10.1016/j.jaci.2012.04.011 Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Oral tolerance protocol. Schematic outline for the feeding and immunization schedule of mice. Mice were immunized and boosted with OVA or CPE, as detailed in the Methods section. Blood was harvested for antibody analysis, or mice were challenged intraperitoneally with 10 mg of OVA or 1 mg of CPE. Journal of Allergy and Clinical Immunology 2012 130, 705-715.e1DOI: (10.1016/j.jaci.2012.04.011) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Mast cells are not required for oral tolerance induction. A, OVA-specific antibody levels were compared between OVA-fed sensitized mice (tol) and control sensitized mice (cont) in C57BL/6 or KitW-sh/W-sh mast cell–deficient mice to determine relative oral tolerance induction. Bars represent mean ± SEM IgE levels or median IgG1 and IgG2a levels with interquartile ranges. B, OVA-specific antibody levels were compared between control and tolerized KitW/W-v mice and control WBB6F1 littermates. C, Anti-peanut antibody levels were compared between peanut butter–fed sensitized mice (tol) and control sensitized mice (cont) from C57BL/6 or KitW-sh/W-sh mice. Bars represent mean ± SEM IgE, IgG1, or IgG2a ODs. *P < .05, **P < .01, and ***P < .001. n.s., Not significant. Journal of Allergy and Clinical Immunology 2012 130, 705-715.e1DOI: (10.1016/j.jaci.2012.04.011) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 IgA tolerance is intact in the absence of mast cells. A, Mice were treated with OVA-containing drinking water (tol) or normal water (cont) for 1 week and then immunized and boosted with OVA, as detailed in Fig 1, A. OVA-specific IgA antibody levels in plasma were compared between tolerized and control KitW-sh/W-sh or C57BL/6 mice 1 week after boost. Bars represent mean ± SEM IgA responses. B, Fecal samples were assessed for OVA-specific IgA in tolerized and control mice. C, OVA-specific IgA levels were compared in plasma of tolerized or control mice 1 week after OVA boost in KitW/W-v mice and WBB6F1 littermates. D, Mice were fed peanut butter (tol) or normal chow (cont) for 1 week and then immunized and boosted with CPE, as detailed in Fig 1, A. Peanut-specific IgA (mean ± SEM) levels were compared between the plasma of tolerized or control mice 1 week after CPE boost in the KitW-sh/W-sh and C57BL/6 groups. *P < .05, **P < .01, and ***P < .001. n.s., Not significant. Journal of Allergy and Clinical Immunology 2012 130, 705-715.e1DOI: (10.1016/j.jaci.2012.04.011) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Physiologically relevant oral tolerance is induced by OVA or peanut butter feeding. Mean core temperature was measured before and 10, 30, and 50 minutes after systemic antigen challenge to assess the effect of oral tolerance on anaphylaxis. A, FcγRIII−/− mice were challenged intraperitoneally with OVA. B, C57BL/6 mice were challenged intraperitoneally with CPE. Average temperatures were compared between groups at each time point by using t tests. Mean ± SEM temperatures are displayed. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001. n.s., Not significant. Journal of Allergy and Clinical Immunology 2012 130, 705-715.e1DOI: (10.1016/j.jaci.2012.04.011) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 OT-II Treg cells are enhanced in the KitW-sh/W-sh MLNs after oral tolerance induction to OVA through a mast cell–independent mechanism. Naive OT-II T cells (1 × 106) were adoptively transferred to C57BL/6, KitW-sh/W-sh, or mast cell–reconstituted (KitW-sh/W-sh recon) mice. Animals were treated with OVA-containing drinking water for 7 days, and the development of Treg cells (Foxp3+) was compared with that seen in untreated control mice. A, Representative plots of CD45.1+/CD4+/Foxp3+ (OT-II Treg cells) cells as a percentage of OT-II CD4+ cells recovered from the MLNs of tolerized or untreated KitW-sh/W-sh mice. B, Transferred OT-II Treg cells recovered from the MLNs and spleen were compared between OVA-tolerized and untreated C57BL/6 or KitW-sh/W-sh mice. C, Tolerized C57BL/6, KitW-sh/W-sh, and KitW-sh/W-sh recon mice were compared by using analysis of OT-II Treg cells as a percentage of OT-II CD4+ T cells recovered from the MLNs or analysis of CD4+ T cells as a percentage of live cells recovered from the PPs. D, Transferred cells were compared in tolerized and untreated C57BL/6 and KitW-sh/W-sh PPs. *P < .05, **P < .01, and ***P < .001. n.s., Not significant. Journal of Allergy and Clinical Immunology 2012 130, 705-715.e1DOI: (10.1016/j.jaci.2012.04.011) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 6 Oral tolerance to OVA in the context of IgE-mediated mast cell activation. A, Mice were sensitized with anti-TNP IgE before treatment with OVA-containing drinking water (tol) or normal water (cont). TNP-BSA or control BSA was delivered by means of intraperitoneal injection during OVA-alum immunization. OVA-specific IgE levels (means ± SEMs) were compared between groups by using ANOVA with the Dunnett multiple comparison test. **P < .01. B, Mice were sensitized with anti-TNP IgE before treatment with OVA-containing drinking water (tol) or normal water (cont). TNP-BSA or control BSA was delivered by means of intraperitoneal injection during gavage treatment with OVA. n.s., Not significant. Journal of Allergy and Clinical Immunology 2012 130, 705-715.e1DOI: (10.1016/j.jaci.2012.04.011) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions