Sanazole as substrate of xanthine oxidase and microsomal NADPH/cytochrome P450 reductase  I.A. Schepetkin, N.V. Cherdyntseva, V.T. Kagiya  Pathophysiology  Volume 8, Issue 2, Pages 119-127 (December 2001) DOI: 10.1016/S0928-4680(01)00073-6

Fig. 1 Effect of sanazole (curves 1 and 3) and metronidazole (curve 2) on the rate of Cyt c3+ reduction in enzyme system xanthine/xanthine oxidase under hypoxygenic (curves 1 and 2) and normoxygenic (curve 3) conditions. Reaction mixtures contained 95 μM xanthine, 2 mU/ml xanthine oxidase, 20 μM Cyt c3+, and indicated concentration of sanazole and metronidazole. The effect was estimated as a magnitude of the ratio of Cyt c3+ reduction for the sample treated with nitro compound to that for the control (no drug treatment); data are averages of three experiments. Pathophysiology 2001 8, 119-127DOI: (10.1016/S0928-4680(01)00073-6)

Fig. 2 Effect of xanthine on the rate of Cyt c3+ reduction ([Cyt c3+]/min) in enzyme system xanthine/xanthine oxidase under hypoxygenic conditions with 8 mM sanazole (curve 1) or without the drug (curve 2). Reaction mixtures contained 2 mU/ml xanthine oxidase, 20 μM Cyt c3+, and indicated concentration of xanthine. Pathophysiology 2001 8, 119-127DOI: (10.1016/S0928-4680(01)00073-6)

Fig. 3 Effect of sanazole concentration (mM−1) on the rate of Cyt c3+ reduction (min mM−1) under hypoxygenic conditions (Lineweaver–Burk plot). The reaction medium contained xanthine (170 μM), xanthine oxidase (2 mU/ml), Cyt c3+ (20 μM), and SOD (50 U/ml). Each point is average of three experiments. Pathophysiology 2001 8, 119-127DOI: (10.1016/S0928-4680(01)00073-6)

Fig. 4 Kinetics of Cyt c3+ reduction in normoxygenic media containing microsomes (100 μg/ml microsomal protein) and Cyt c3+ (20 μM) after the addition of NADPH (100 μM) (indicated by arrow) (curve 1) or NADPH (100 μM) and SOD (50 U/ml) (curve 2); curves 3 and 4 are the same as curves 1 and 2, accordingly, but in the presence of 8 mM sanazole. Pathophysiology 2001 8, 119-127DOI: (10.1016/S0928-4680(01)00073-6)

Fig. 5 Kinetics of Cyt c3+ reduction in hypoxygenic (curves 1, 3, and 5) and normoxygenic (curves 2 and 4) media containing xanthine oxidase (2 mU/ml), Cyt c3+ (20 μM), and SOD (50 U/ml) after the addition of xanthine (95 μM) (indicated by arrow) and 150 μl of the buffer (curves 1 and 2) or a nitroazole compound (in 150 μl): metronidazole (final concentration 6 mM) (curve 3) or sanazole (final concentration 7 mM) (4 and 5); 6 is the same as 5 but the reaction was performed in the presence of catalase (5000 U/ml). Pathophysiology 2001 8, 119-127DOI: (10.1016/S0928-4680(01)00073-6)

Fig. 6 Absorption spectra of sanazole (curve 1) and its reduced products 4 min after the addition of zinc powder (5 mg/ml) to 100 μM solution of sanazole in 2 mM (curve 2), 4 mM (curve 3), or 10 mM (curve 4) solutions of NH4Cl. Pathophysiology 2001 8, 119-127DOI: (10.1016/S0928-4680(01)00073-6)