Is Tracheal Transplantation Possible With Cryopreserved Tracheal Allograft and Hyperbaric Oxygen Therapy? An Experimental Study Fatih Candas, MD, Rauf.

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Is Tracheal Transplantation Possible With Cryopreserved Tracheal Allograft and Hyperbaric Oxygen Therapy? An Experimental Study Fatih Candas, MD, Rauf Gorur, MD, Aptullah Haholu, MD, Akin Yildizhan, MD, Orhan Yucel, MD, Hakan Ay, MD, Ali Memis, MD, Turgut Isitmangil, MD The Annals of Thoracic Surgery Volume 101, Issue 3, Pages 1139-1144 (March 2016) DOI: 10.1016/j.athoracsur.2015.09.018 Copyright © 2016 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 (A) Fresh tracheal epithelium, (B) exfoliated epithelium cryopreserved tracheal allograft (CTA), (C) transplanted CTA in rat showing a macroscopic view of the excised CTA, (D) early period, and (E) late period (hematoxylin and eosin stain; A and B: original magnification × 200). The Annals of Thoracic Surgery 2016 101, 1139-1144DOI: (10.1016/j.athoracsur.2015.09.018) Copyright © 2016 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Histologic examination in the early period of (A and B) control and (C and D) hyperbaric oxygen treatment groups shows no significant histopathologic differences between the groups (hematoxylin and eosin stain; A and C: original magnification ×40; B and D: original magnification ×100). The Annals of Thoracic Surgery 2016 101, 1139-1144DOI: (10.1016/j.athoracsur.2015.09.018) Copyright © 2016 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 Histologic examination in the late period. (A and B) Epithelization is visible only in the anastomotic region in the control group. (C and D) Native tracheal epithelium and the allograft epithelium in the hyperbaric oxygen treatment group. A ‘‘competition’’ between epithelial regeneration and fibroblastic proliferation results in (A) tracheal stenosis at control group, (C) and lumen patency in the HBOT group (hematoxylin and eosin stain; A and C: original magnification ×40; B and D: original magnification ×100). The Annals of Thoracic Surgery 2016 101, 1139-1144DOI: (10.1016/j.athoracsur.2015.09.018) Copyright © 2016 The Society of Thoracic Surgeons Terms and Conditions