M. Montes, D. Vicente, O. Esnal, G. Cilla, E. Pérez-Trallero

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A PCR–restriction fragment length polymorphism assay to genotype human metapneumovirus M. Montes, D. Vicente, O. Esnal, G. Cilla, E. Pérez-Trallero Clinical Microbiology and Infection Volume 14, Issue 1, Pages 91-93 (January 2008) DOI: 10.1111/j.1469-0691.2007.01875.x Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Typing of human metapneumovirus (hMPV) according to fusion gene PCR–restriction fragment length polymorphism (PCR-RFLP) analysis. A partial fusion gene was amplified by PCR and analysed following digestion with Tsp509I. Detection of hMPV generated a 348-bp product. Lanes: 1 and 15, 100-bp DNA molecular size ladder (Tracklt; Invitrogen, Carlsbad, CA, USA); 2 and 3, hMPV lineage B1; 4 and 5, hMPV lineage B2; 6–8, hMPV lineage A1; 9–11, hMPV lineage A2a; 12–14, hMPV lineage A2b. Clinical Microbiology and Infection 2008 14, 91-93DOI: (10.1111/j.1469-0691.2007.01875.x) Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions