Volume 52, Issue 2, Pages 455-463 (August 2007) Polycomb-Group Oncogenes EZH2, BMI1, and RING1 Are Overexpressed in Prostate Cancer With Adverse Pathologic and Clinical Features  Geert J.L.H. van Leenders, Danny Dukers, Daphne Hessels, Susan W.M. van den Kieboom, Christina A. Hulsbergen, J. Alfred Witjes, Arie P. Otte, Chris J. Meijer, Frank M. Raaphorst  European Urology  Volume 52, Issue 2, Pages 455-463 (August 2007) DOI: 10.1016/j.eururo.2006.11.020 Copyright © 2006 European Association of Urology Terms and Conditions

Fig. 1 Expression of PcG proteins in normal prostate epithelium. EZH2 is sporadically found in normal prostate epithelium (arrow). The PcG proteins YY1, BMI1, RING1, and HPH1 are abundantly expressed in both basal and luminal compartments. HPC2 expression is restricted to a majority of luminal cells and rare basal cells. (Original magnification: ×200). European Urology 2007 52, 455-463DOI: (10.1016/j.eururo.2006.11.020) Copyright © 2006 European Association of Urology Terms and Conditions

Fig. 2 Expression of PcG proteins in prostate adenocarcinoma. The protein EZH2 is expressed in a minor subpopulation of malignant cells, whereas YY1, BMI1, RING1, HPC2, and HPH1 are abundantly present. (Original magnification: ×200). European Urology 2007 52, 455-463DOI: (10.1016/j.eururo.2006.11.020) Copyright © 2006 European Association of Urology Terms and Conditions

Fig. 3 Disease-free survival in low-grade prostate adenocarcinoma (Gleason score ≤6). (Left) Patients with a median EZH2 expression of ≥1% (bracket line) have a significantly shorter disease-free survival compared with patients with low (<1%) expression (continuous line) (p=0.013). (Right) Patients with a moderate to strong median BMI1 expression (bracket line) have a worse prognosis than patients with weak or negative expression (continuous line) (p=0.046). European Urology 2007 52, 455-463DOI: (10.1016/j.eururo.2006.11.020) Copyright © 2006 European Association of Urology Terms and Conditions

Fig. 4 Expression of EZH2 in prostate cancer metastasis. (Left) Follicle centers highly express EZH2 and serve as an internal control (EZH2-negative tumor area is shown on the right side). (Right) EZH2 only occurs at very low levels within prostate cancer metastasis. (Original magnification: ×200). European Urology 2007 52, 455-463DOI: (10.1016/j.eururo.2006.11.020) Copyright © 2006 European Association of Urology Terms and Conditions