iGEM 2005 & 2006 http://arep.med.harvard.edu/SBP/iGEM/iGEM_2004.ppt http://bio.freelogy.org/wiki/IGEM2005 http://openwetware.org/wiki/IGEM:Harvard/2006 http://openwetware.org/wiki/IGEM:Harvard/2007

2007 Pre-iGEM meetings * Overview * Parts & Devices * Case study (& Voigt review) * New Strengths 2: list of working parts & systems 3. Brainstorm projects 4. Show how to design & order parts.

Biobricks Proposal that the 2007 iGEM awards be biological, useful, & CO2-neutral.

The 2004 iGEM poster was synthetic, but not biological ( an artifact of light sensitive dyes) Privately rehabilitated in time for 2005 iGEM jamboree & then publication: Levskaya A, et al. Nature 2005 Nov 24 Synthetic biology: engineering E.coli to see light. Low contrast: Input: Red light / phycocyanobilin Out: LacZ / S-gal (3,4-cyclohexenoesculetin- -D-galactopyranoside):

Voigt 2006 Current Opinion in Biotechnology 2006, 17:548–557 Light Input/Output Voigt 2006 Current Opinion in Biotechnology 2006, 17:548–557

Possible alternatives for light I/O ‘Caged’ substrates for light input Multiple wavelengths Luciferase output

Potential strengths barely tapped Combinatorial constructions Selections Surface expression Homologous recombination

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