The biology of the skeleton

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Presentation transcript:

The biology of the skeleton

Bone is more complex than you might think! Some of the many functions of bone: -Protection of organs -Mineral storage -Making red and white blood cells -MOVEMENT!

What kinds of bone are there? Cortical bone Spongy

What is bone made of? Mineral (calcium phosphate) Organic molecules (like type I collagen) CELLS Osteoblasts Osteoclasts Osteocytes

Osteoblasts that became trapped in the newly made bone. Bone cells “Osteo” means “bone” in Greek Bone cells are responsible for forming and remodeling your skeleton Osteocytes Osteoclasts Osteoblasts Bone eating cells Bone forming cells Osteoblasts that became trapped in the newly made bone. new bone Bone Surface Bone Surface Bone Surface

Your bones require MECHANICAL LOAD to stay strong http://www.ice.org/unzipped/2002/11/dv-astronaut.jpg http://msnbcmedia.msn.com/j/msnbc/Components/Photos/050125/050125_federer_agassi_hmed_5a.hmedium.jpg

What happens when your bones AREN’T strong? Osteoclasts are doing more work than osteoblasts! The osteoclasts are breaking down more bone than osteoblasts are making. Bone resorption > Bone formation BONE LOSS!!!

How can we FIX osteoporosis? Bone resorption > Bone Formation BONE LOSS Osteoporosis! Bone resorption < Bone Formation osteoclast osteoblast BONE GAIN

Does the behavior of cells in the bone change depending on what surrounds it? Can we force osteoblasts to make MORE BONE by changing the surroundings of the cell?

Techniques I use… Cell culture Microscopy Calcium imaging Atomic Force Microscopy Confocal Microscopy Calcium imaging Reporter assays Confocal Microscopy AFM Calcium Imaging

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