Dose-dependent effect of lipopolysaccharide (LPS) on neutrophil-induced trans-basement membrane migration of eosinophils in severe asthmatics. Dose-dependent.

Slides:

Advertisements

Similar presentations

Fig. 2 Effect of resistin and bFGF on human endothelial cells by MTS assay. A. Serum-starved HCAECs were treated with measured doses of resistin (0–80.

Advertisements

Cell Physiol Biochem 2016;40: DOI: /

Fig. 4. Migration assays in neonatal normal human keratinocytes (NHKs) and human dermal fibroblasts (HDFs). NHKs (A) and HDFs (B) were cultured in either.

Volume 122, Issue 2, Pages (February 2002)

Fig. 3. ATME inhibits VEGF-induced invasion and tube formation of endothelial cells. (A) Effect of ATME on HUVEC invasion by using the Transwell culture.

Hon-Wai Koon, Dezheng Zhao, Hua Xu, Collin Bowe, Alan Moss, Mary P

A stepwise therapeutic approach in severe uncontrolled asthmatic subjects. A stepwise therapeutic approach in severe uncontrolled asthmatic subjects. GINA:

Effect of pre-treatment with lipopolysaccharide (LPS) or cystic fibrosis (CF) serum on the production of reactive oxygen species (ROS) by phagocytes from.

Specificity of production of reactive oxygen species (ROS) against Aspergillus fumigatus conidia by phagocytes from healthy and CF donors. Specificity.

The interaction of human peripheral blood eosinophils with bacterial lipopolysaccharide is CD14 dependent by Sabine G. Plötz, Arnd Lentschat, Heidrun Behrendt,

Lipopolysaccharide Activates Caspase-1 (Interleukin-1–Converting Enzyme) in Cultured Monocytic and Endothelial Cells by Ralf R. Schumann, Claus Belka,

Metalloproteinases Are Involved in Lipopolysaccharide– and Tumor Necrosis Factor-–Mediated Regulation of CXCR1 and CXCR2 Chemokine Receptor Expression.

Adrian Schreiber, Friedrich C. Luft, Ralph Kettritz

Echocardiography view

Dose-dependent effect of lipopolysaccharide (LPS) on neutrophil-induced trans-basement membrane migration of eosinophils in healthy volunteers. Dose-dependent.

Compliance of the respiratory system (Crs) at baseline and 0 h, 1 h and 2 h following lung volume recruitment. a) Absolute Crs in the respiratory muscle.

Bland–Altman plots of in vitro functional residual capacity (FRC) measurements on each device. Bland–Altman plots of in vitro functional residual capacity.

The correlation between Mycobacterium growth indicator tube (MGIT) time to culture positivity (TTP) and bacterial load count by molecular bacterial load.

Secretion of monocyte chemotactic protein-1 by human uterine epithelium directs monocyte migration in culture Richard A. Meter, M.D., Charles R. Wira,

Effect of caspase-1 deficiency on lung inflammatory parameters and inflammatory cell subsets in a mouse model of asthma exacerbation. Effect of caspase-1.

Bland–Altman plot of slopes of measurement 1 versus 2

Change from baseline following beclomethasone/formoterol and placebo in a) platelet–monocyte aggregate formation, b) P-selectin expression and c) platelet.

Volume 124, Issue 1, Pages (January 2003)

Effects of BAs on BEAS-2B cell viability.

Gemifloxacin inhibits cytokine secretion by lipopolysaccharide stimulated human monocytes at the post-transcriptional level F. Araujo, T. Slifer, S.

Lung interleukin (IL)-33 protein expression in vivo depends on caspase-1. Lung interleukin (IL)-33 protein expression in vivo depends on caspase-1. Mice.

Caspase-1 deficient mice have a lower expression of lung T-helper type 2 (Th2)-upstream cytokines at exacerbation. Caspase-1 deficient mice have a lower.

Venn diagram showing the overlap between the various chronic obstructive pulmonary disease (COPD) diagnoses: self-reported physician-diagnosed, fixed ratio.

A B C D ID8 vector scr sh cx2 cx3 cx4 cx6 CX3CR1 β-tubulin * ID8

A) White blood cell (WBC) counts for 10 lymphangioleiomyomatosis (LAM) patients (nine on sirolimus, one off sirolimus) experiencing pseudoneutropenia in.

E. Boulanger, N. Grossin, M.-P. Wautier, R. Taamma, J.-L. Wautier

Induction of the nuclear factor (erythroid derived 2)-like 2 antioxidant response element (Nrf2-ARE) target genes in mesenchymal stromal cells (MSCs) from.

Volume 77, Issue 2, Pages (January 2010)

Rhinovirus (RV) infection induces caspase-1 expression in bronchial epithelial cells in asthma. Rhinovirus (RV) infection induces caspase-1 expression.

Comparison of absolute counts for a, b) white blood cells (WBC), c, d) neutrophils, e, f) monocytes, and g, h) eosinophils for 100 patients with lymphangioleiomyomatosis.

Rolipram Inhibits Polarization and Migration of Human T Lymphocytes

Leukotriene B4 (LTB4) levels in asthmatic patients when stratified according to the sputum cellularity. Leukotriene B4 (LTB4) levels in asthmatic patients.

Taeseung Lee, MD, Nowokere Esemuede, MD, Bauer E

Average mean arterial pressure (Pa) during lung volume recruitment (LVR) in (a) the respiratory muscle weakness (RMW) group and (b) the control group.

Individual plots of overall Standardised Asthma Quality of Life Questionnaire (AQLQ(S)) scores in each Global Initiative for Asthma level of asthma control.

Toll-like receptor (TLR) 2-induced inflammatory signalling is more resistant to dexamethasone than TLR5-driven signalling. a) BEAS-2B NF-κB luciferase.

Scatter plot of the % change versus baseline in pulmonary function test results at a) 4 months and b) the end of the study versus the analyte concentration.

Venn diagram showing the overlap between the various spirometric definitions of airway obstruction and respiratory symptoms. **: p

Measurement of a) IL-8, b) IL-6 and c) GM-CSF release by BEAS-2B cells stimulated with deoxycholic acid for 48 h. Measurement of a) IL-8, b) IL-6 and c)

Volume 53, Issue 6, Pages (June 1998)

Individual plots of Standardised Asthma Quality of Life Questionnaire (AQLQ(S)) scores for the symptoms domain in each Global Initiative for Asthma level.

Individual plots of Standardised Asthma Quality of Life Questionnaire (AQLQ(S)) scores for the activity limitation domain in each Global Initiative for.

Blazej Zbytek, Andrzej T. Slominski

Volume 6, Issue 4, Pages (April 2016)

Effect of PDGF-BB on mesenchymal mesonephric cell migration.

Fig. 1. Treatment with IL-13 induces proliferation and migration of BSMCs. (A) MTT assay was performed to evaluate the effects of IL-13 on the proliferation.

Effect of IBMP and PKG on cAMP levels

Post-operative a) neutrophil counts, but not b) lymphocyte or c) platelet counts, were higher in current smokers than ex-smokers. #: time-points were statistically.

Volume 56, Issue 3, Pages (September 1999)

Jason G. Umans, Debra Salvi, Patrick T. Murray, Mark E. Wylam

Innate Immune Recognition of Bacterial Lipopolysaccharide: Dependence on Interactions with Membrane Lipids and Endocytic Movement Nathalie Thieblemont,

Pro-proliferative effect of lipoxin A4 (LXA4) is phosphatidylinositol 3′-kinase dependent and mediated by ALX/formyl peptide L1 receptor. 100 nM LXA4 promoted.

Primary human airway smooth muscle cells were stimulated with different concentrations of a, e) 18 mg·mL−1 nicotine tobacco-flavoured e-cigarette vapour.

IL8 and TNFα are required for neutrophil-induced UMSCC47 increase in invasion. IL8 and TNFα are required for neutrophil-induced UMSCC47 increase in invasion.

Neutrophils increase UMSCC47 invasion.

VEGF165 stimulates migration of HMVECs

Inhibitory effects of nobiletin and luteolin on TPA-induced O2− generation in differentiated HL-60 cells and their cellular uptake. Inhibitory effects.

Effect of microRNA (miR)-17-loaded lipid–polymer hybrid nanoparticles (LPNs) on interleukin (IL)-8 expression. Effect of microRNA (miR)-17-loaded lipid–polymer.

KIR2DL4 on human mast cells upregulates the invasive activity of HLA-G+ cancer cell lines. KIR2DL4 on human mast cells upregulates the invasive activity.

Results of the murine forced swim test and spontaneous locomotor activity tests for ivacaftor, fluoxetine and ketamine. a) Schematic diagram depicting.

Increased invasion does not require direct contact between neutrophils and UMSCC47. Increased invasion does not require direct contact between neutrophils.

Effect of PD on LPS-induced activation of ERK1 and ERK2 in human monocytes. Effect of PD on LPS-induced activation of ERK1 and ERK2 in human.

T (min) FSK-treated IBM-X-treated A B C ns * **** ** Figure S3.

Effect of lipoxin A4 (LXA4) on alveolar type II cell (ATII) wound repair. Effect of lipoxin A4 (LXA4) on alveolar type II cell (ATII) wound repair. LXA4.

Effect of inhibition of phagocytosis on the production of reactive oxygen species (ROS) against Aspergillus fumigatus. Effect of inhibition of phagocytosis.

Presentation transcript:

Dose-dependent effect of lipopolysaccharide (LPS) on neutrophil-induced trans-basement membrane migration of eosinophils in severe asthmatics. Dose-dependent effect of lipopolysaccharide (LPS) on neutrophil-induced trans-basement membrane migration of eosinophils in severe asthmatics. Neutrophils (2×104 cells) from severe asthmatics were stimulated with various concentrations of LPS (0.01–100 µg·mL−1), and then placed into the lower compartment of a chamber with a Matrigel-coated Transwell insert. Eosinophils (1×105 cells) from severe asthmatics were added to the upper compartment. After 120 min of incubation, migrated eosinophils in the lower chamber were measured by eosinophil peroxidase assays (n=4). Data are presented as mean±sem. *: p<0.05 versus spontaneous migration (0 µg·mL−1 LPS) by Tukey test; **: p<0.01 versus spontaneous migration (0 µg·mL−1 LPS) by Tukey test. European Respiratory Society ERJ Open Res 2017;3:00003-2015-AUT ©2017 by European Respiratory Society