Lab Meeting 10-18-13. Tet Tet+ 0.1mM Caffeine Tet+ 0.1mM Theophylline Results of Single Colony That Grew on Tet + Caffeine Grown at 25C for 4 days.

Slides:



Advertisements
Similar presentations
TWO STEP EQUATIONS 1. SOLVE FOR X 2. DO THE ADDITION STEP FIRST
Advertisements

Numbers Treasure Hunt Following each question, click on the answer. If correct, the next page will load with a graphic first – these can be used to check.
Objective - To use basic trigonometry to solve right triangles.
Warm Up Problem of the Day Lesson Presentation Lesson Quizzes.
There is a pattern for factoring trinomials of this form, when c
Supplemental Web Fig. 2 mos10/mos10, grown on 100 mm plate SLAS27 medium, day 1.
Supplemental Web Fig. 1 Wild type, grown on 100 mm plate SLAS27 medium, day 1.
© 2008 Pearson Addison Wesley. All rights reserved Chapter Seven Costs.
Chapter 1 The Study of Body Function Image PowerPoint
Socratic seminar 13- end, collect SR, LL conference HW: quiz over Monday: Socratic seminar 13-end, collect SR, LL conference Wednesday: ACT day,
Fractions VI Simplifying Fractions
and 6.855J Cycle Canceling Algorithm. 2 A minimum cost flow problem , $4 20, $1 20, $2 25, $2 25, $5 20, $6 30, $
Jeopardy Q 1 Q 6 Q 11 Q 16 Q 21 Q 2 Q 7 Q 12 Q 17 Q 22 Q 3 Q 8 Q 13
Jeopardy Q 1 Q 6 Q 11 Q 16 Q 21 Q 2 Q 7 Q 12 Q 17 Q 22 Q 3 Q 8 Q 13
Title Subtitle.
Determine Eligibility Chapter 4. Determine Eligibility 4-2 Objectives Search for Customer on database Enter application signed date and eligibility determination.
Finding a Common Denominator
Reducing Fractions. Factor A number that is multiplied by another number to find a product. Factors of 24 are (1,2, 3, 4, 6, 8, 12, 24).
Frigid Fridays On each Friday last January, the temperature was a record low temperature for the date. On Friday, January 30, the mercury dropped to 5°F,
0 - 0.
1  1 =.
2 pt 3 pt 4 pt 5 pt 1 pt 2 pt 3 pt 4 pt 5 pt 1 pt 2 pt 3 pt 4 pt 5 pt 1 pt 2 pt 3 pt 4 pt 5 pt 1 pt 2 pt 3 pt 4 pt 5 pt 1 pt Time Money AdditionSubtraction.
Hosted By Prissy & Stephy Multiplying exponents Negative exponents Dividing exponent percents
MULTIPLYING MONOMIALS TIMES POLYNOMIALS (DISTRIBUTIVE PROPERTY)
FACTORING ax2 + bx + c Think “unfoil” Work down, Show all steps.
Addition Facts
Year 6 mental test 5 second questions
Year 6 mental test 10 second questions
It is 2 o C The temperature drops by 3 degrees What temperature is it now? -1 o C.
Welcome to Who Wants to be a Millionaire
SOLVING EQUATIONS AND EXPANDING BRACKETS
1 Like Terms Press Ctrl-A ©2009 G Dear – Not to be sold/Free to use Stage 4 Years 7 & 8.
ABC Technology Project
1 Undirected Breadth First Search F A BCG DE H 2 F A BCG DE H Queue: A get Undiscovered Fringe Finished Active 0 distance from A visit(A)
VOORBLAD.
1 Breadth First Search s s Undiscovered Discovered Finished Queue: s Top of queue 2 1 Shortest path from s.
Factor P 16 8(8-5ab) 4(d² + 4) 3rs(2r – s) 15cd(1 + 2cd) 8(4a² + 3b²)
Linking Verb? Action Verb or. Question 1 Define the term: action verb.
SYSTEMS OF EQUATIONS.
8 2.
Revision - Simultaneous Equations II
© 2012 National Heart Foundation of Australia. Slide 2.
Past Tense Probe. Past Tense Probe Past Tense Probe – Practice 1.
Understanding Generalist Practice, 5e, Kirst-Ashman/Hull
Grade 2 Created by; Kim Smith
GG Consulting, LLC I-SUITE. Source: TEA SHARS Frequently asked questions 2.
Addition 1’s to 20.
Start. 5a - 5 = 7a - 19 A. 7 B. 16 C. -7 D. None of these.
25 seconds left…...
Factoring Grouping (Bust-the-b) Ex. 3x2 + 14x Ex. 6x2 + 7x + 2.
Test B, 100 Subtraction Facts
Januar MDMDFSSMDMDFSSS
Week 1.
We will resume in: 25 Minutes.
©Brooks/Cole, 2001 Chapter 12 Derived Types-- Enumerated, Structure and Union.
Algebra I B. MacDonald.  What is a GCF? What is a GCF?  Factoring out a GCF Factoring out a GCF.
Bottoms Up Factoring. Start with the X-box 3-9 Product Sum
PSSA Preparation.
1 Chapter 13 Nuclear Magnetic Resonance Spectroscopy.
1.6 – Solving Compound and Absolute Value Inequalities
Lesson 2-3 Example Example 1 Use the Distributive Property and a model to find 6 ● Draw a model to show 6 ● 13.
Lecture 16 chi-square test (continued) Suppose 160 pairs of consecutive nucleotides are selected at random. Are data compatible with the independent occurrence.
Presidential Fitness Program Pull/Push upMile RunShuttle RunSit-UpsV-Sit Stretch.
Caffeine Disk Results Spring Procedure Grow clone from glycerol stock – Amp or amp + chlor – 4 mM caffeine Measure A590 and standardize to 0.1 or.
E. Coli response to Theophylline and Caffeine. Procedure Using a plate reader absorbance was measured of J11940+J119303, J J119304, J11940 in pSB4c5,
The reading is 7.38 mm. The reading is 7.72 mm.
روش تحقيق بارويكرد ي به پايان نامه نويسي
مديريت موثر جلسات Running a Meeting that Works
Presentation transcript:

Lab Meeting

Tet Tet+ 0.1mM Caffeine Tet+ 0.1mM Theophylline Results of Single Colony That Grew on Tet + Caffeine Grown at 25C for 4 days

Results: ecdm8 + TetA Tet Tet+ 0.1mM Caffeine Tet + 0.1mM Theophylline Grown at 25C for 4 days

Drops of Theo or Caffeine on J303/4C5 + J79/1A2

Disks of Theo or Caffeine on J303/1A2 + J79/4C5

Disks of Theo or Caffeine on J303/4C5 + J79/1A2

30 Degrees C

Next Steps Continue Running Experiment at 30C for 2 days Finishing cloning ecdm8 downstream of TetA