HIV Testing Algorithm HIV Ag Ab with Reflex (>2 y/o and older)*

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HIV Testing Algorithm HIV Ag Ab with Reflex (>2 y/o and older)* (HIVSC) * For HIV-1 RNA Qualitative detection on pediatric patients < 24 months of age, order: HIVQL, specimen: PLASMA, lavender top EDTA (specimen vol. 1 mL, min 0.5 mL) *For HIV-1 DNA and RNA Qualitative detection on pediatric patients <18 months of age, order: HIVPS, specimen: PLASMA, lavender top EDTA (specimen vol. 1 mL) Non-reactive (no further testing) Reactive HIV-1 Ag and/or HIV-1 Ab Reactive HIV-2 Ab Reflex to HIV1/2 Ab Supplemental/Differentiation (HIVSD) * Consider HIV-2 DNA/RNA send out based on patient’s exposure history, order in EPIC “Miscellaneous Lab Request,” indicate a send out test to Mayo with code FHV2Q. Both HIV-1 and HIV-2 negative* HIV-1 positive or indeterminate HIV-2 positive or indeterminate Reflex to HIV-1 RNA Viral Load (VILO) Reflex to HIV-2 DNA/RNA send out (FHV2Q) Detected Undetected Detected Undetected HIV-1 Infection Repeat HIV Ag/Ab (HIVSC) in 2-4 weeks for high-risk patients HIV-2 Infection Repeat HIV Ag/Ab (HIVSC) in 2-4 weeks for high risk patients Last Updated: October 2018