Study of Lignin Degradation in soil

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Presentation transcript:

Study of Lignin Degradation in soil Lab Meeting -15th may 2009 Presentation by Mythreyi Chandoor Directed by Prof. Dr. Shulin Chen

Isolation of microcosm from Soil and Biomass samples Isolating the mixed Cultures and plating them on LB + Azodyes,(0.002 concentration).

Sample 1 Sample 2 Sample 4 sample 5 Sample 6 sample 13 sample 12 647nm TIMING concentration od 4 hours 4.458 7.315 11.373 5.812 7.048 8.274 3.455 0.1 0.506 8 hours 10.904 8.868 3.982 4.412 10.109 9.559 8.977 0.5 0.934 12 hours 4.927 12.293 6.493 9.817 6.7 11.326 12.695 1 1.722 16 hours 1.717 0.2 5.214 10.204 5.108 0.064 11.023 5 2.564 20 hours 4.94 4.697 7.117 4.565 5.646 9.494 12.238 10 3.793 24 hours 8.276 11.398 6.45 11.191 8.846 10.028 11.873 OD OD values Standard 1.498 1.277 0.964 1.393 1.298 1.203 1.576 1.157 1.535 1.285 1.061 1.104 1.149 1.462 0.893 1.341 1.084 1.273 0.967 0.862 1.679 1.809 1.054 1.404 1.837 0.746 1.461 1.48 1.293 1.49 1.406 1.109 0.897 0.962 1.344 0.978 1.159 1.068 0.925 Observation using UV-Spectrophotometry of the Discoloration of Azodye in LB liquid media for a set of every 4 hours for 24 hours.

OD values Concentration versus Time Future work is to determine the structural changes in lignin structure of the wood and wheat straw samples from NMR,GC-MS analysis.