Introduction to the Microscope

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Introduction to the Microscope

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Presentation transcript:

Introduction to the Microscope Types of Microscopes Care Parts Focusing Students will have their microscopes out, for reference as we go through each of the steps. As an introduction, students will be asked what kinds of things they can do with this tool.

Types of Microscopes Light Microscope - the models found in most schools, use compound lenses to magnify objects. The lenses bend or refract light to make the object beneath them appear closer. Common magnifications: 40x, 100x, 400x *Oil Immersion lenses can improve quality of focus and magnification

Stereoscope This microscope allows for binocular (two eyes) viewing of larger specimens. Usually magnifies 10x to 20x Can be used for thicker specimens Creates a 3D view of specimen

Scanning Electron Microscope - allows scientists to view a universe too small to be seen with a light microscope. SEMs do not use light waves; they use electrons (negatively charged electrical particles) to magnify objects up to two million times. SEM creates a 3D view of specimen, but cannot view living specimens (process kills them)

Transmission Electron Microscope - also uses electrons, but instead of scanning the surface (as with SEM's) electrons are passed through very thin specimens. TEM = "thin"

TEM of a cell, notice you see the inside of the cell and not the surface.

Types of Illumination in Microscopes How light passes through a specimen changes the view of the specimen, making some parts more distinct. Figure 4B

Eyepiece Body Tube Revolving Nosepiece Arm Objective Lens Stage This is the exact version of the microscope used in class. Students will be identifying the parts on the microscopes at their desks as we go along and what their functions are. Stage Stage Clips Coarse Focus Diaphragm Fine Focus Light Base

Magnification Your microscope has 3 magnifications: Scanning, Low and High. Each objective will have written the magnification. In addition to this, the ocular lens (eyepiece) has a magnification. The total magnification is the ocular x objective

Microscope Resources Virtual Microscopes (Phase Contrast, Fluorescence, TEM, STM) at http://nobelprize.org/educational/physics/microscopes/1.html Nanoworld Image Gallery Microworld Images