Effects of glucosamine sulfate on intracellular UDP-hexosamine and UDP-glucuronic acid levels in bovine primary chondrocytes C.-J. Qu, M.D., M. Jauhiainen,

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Presentation transcript:

Effects of glucosamine sulfate on intracellular UDP-hexosamine and UDP-glucuronic acid levels in bovine primary chondrocytes C.-J. Qu, M.D., M. Jauhiainen, B.Sc., S. Auriola, Ph.D., H.J. Helminen, Ph.D., M.J. Lammi, Ph.D. Osteoarthritis and Cartilage Volume 15, Issue 7, Pages 773-779 (July 2007) DOI: 10.1016/j.joca.2007.01.010 Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 The biosynthetic pathway of CS from glucose or glucosamine. UDP-Glc, UDP-glucose; Acetyl-CoA, acetyl-coenzymeA; UDP-GlcNAc, UDP-N-acetylglucosamine; UDP-GalNAc, UDP-N-acetylgalactosamine; GFAT, glutamine:fructose-6-phosphate aminotransferase; UDP-GlcA, UDP-glucuronic acid. Osteoarthritis and Cartilage 2007 15, 773-779DOI: (10.1016/j.joca.2007.01.010) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 The levels of UDP-Hex (black bar), UDP-GlcA (white bar) and UDP-HexN (grey bar) analyzed by RP-HPLC/ESI-MS after bovine primary chondrocytes were treated with 0, 10, 100μM and 1mM of glucosamine (GlcN) (A) and glucosamine sulfate (GS) in low-glucose DMEM (B) for 10min. The experiments were performed with cell cultures from three different animals. Osteoarthritis and Cartilage 2007 15, 773-779DOI: (10.1016/j.joca.2007.01.010) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 The levels of UDP-Hex (black bar), UDP-GlcA (white bar) and UDP-HexN (grey bar) analyzed by RP-HPLC/ESI-MS measurements after the chondrocytes were treated with 1mM of GS in low-glucose DMEM (A) for 0, 10, 20, 30, 60 and 120min, and with 10μM of GS in low-glucose DMEM for 30 and 60min (B). The experiments were performed with cell cultures from two and nine different animals, respectively. Osteoarthritis and Cartilage 2007 15, 773-779DOI: (10.1016/j.joca.2007.01.010) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 The effects of different concentrations of GS on the intracellular levels of UDP-Hex (black bar), UDP-GlcA (white bar) and UDP-HexN (grey bar) analyzed by RP-HPLC/ESI-MS measurements after the bovine primary chondrocytes were treated with 0, 10μM and 1mM of GS for 30min following 2-day-long cell culture period (A) or 8-day-long cell culture period (B) after cell isolation. *P<0.05. The experiments were repeated with chondrocytes from six different animals. Osteoarthritis and Cartilage 2007 15, 773-779DOI: (10.1016/j.joca.2007.01.010) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions