Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates Chun-Chia Huang, M.Sc., David.

Slides:

Advertisements

Similar presentations

A review and meta-analysis of prospective trials comparing different catheters used for embryo transfer William M. Buckett, M.D. Fertility and Sterility

Advertisements

Akanksha Mehta, M. D. , Alexander Bolyakov, M. S. , Peter N

Elias M. Dahdouh, M. D. , M. Sc. , Jacques Balayla, M. D

Demián Glujovsky, M.D., M.Sc., Cynthia Farquhar, M.D., M.P.H.

Ratio between inner cell mass diameter and blastocyst diameter is correlated with successful pregnancy outcomes of single blastocyst transfers Miriam.

Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study Ashok Agarwal, Ph.D., Fnu Deepinder, M.D., Marcello.

Marcos Meseguer, Ph. D. , Rebeca Santiso, Ph. D. , Nicolas Garrido, Ph

Local injury to the endometrium in controlled ovarian hyperstimulation cycles improves implantation rates Liang Zhou, M.D., Rong Li, M.D., Ph.D., Rong.

DNA fragmentation of human sperm can be detected by ligation-mediated real-time polymerase chain reaction Jung Jin Lim, Ph.D., Jin Il Lee, M.Sc., Dong.

Sperm chromatin structure assay parameters are not related to fertilization rates, embryo quality, and pregnancy rates in in vitro fertilization and intracytoplasmic.

The relative effects of hormones and relationship factors on sexual function of women through the natural menopausal transition Lorraine Dennerstein,

Michael M. Alper, M.D. Fertility and Sterility

Sperm DNA damage measured by the alkaline Comet assay as an independent predictor of male infertility and in vitro fertilization success Luke Simon,

Relationship between pre-embryo pronuclear morphology (zygote score) and standard day 2 or 3 embryo morphology with regard to assisted reproductive technique.

Marcos Meseguer, Ph. D. , Rebeca Santiso, Ph. D. , Nicolás Garrido, Ph

Hee-Jun Chi, Ph. D. , Jung-Jin Koo, M. D. , Soon-Young Choi, M. Sc

Can an educational DVD improve the acceptability of elective single embryo transfer? A randomized controlled study Nicole Hope, M.B., B.S., Hon., Luk.

Effects of retinoic acid on the inner cell mass in mouse blastocysts

Sperm deoxyribonucleic acid fragmentation dynamics in fertile donors

Comparison of fallopian tube sperm perfusion and intrauterine tuboperitoneal insemination: a prospective randomized study Leonidas Mamas, M.D., Ph.D.

Sperm head morphology is related to high deoxyribonucleic acid stainability assessed by sperm chromatin structure assay Armand Zini, M.D., Simon Phillips,

Evaluation of the immature human oocytes from unstimulated cycles in polycystic ovary syndrome patients using a novel scoring system Shan Liu, M.D.,

Medical treatment of ectopic pregnancy: a committee opinion

The relative contribution of assisted reproductive technologies and ovulation induction to multiple births in the United States 5 years after the Society.

Live birth of twins derived from zona-free oocytes

Double probing individual human spermatozoa: aniline blue staining for persistent histones and fluorescence in situ hybridization for aneuploidies Laszlo.

Ying-Hung Lin, Ph. D. , Chuan-Kai Chou, M. Sc. , Yu-Ching Hung, M. Sc

Association between socio-psycho-behavioral factors and male semen quality: systematic review and meta-analyses Ying Li, Ph.D., Hui Lin, Ph.D., Yafei.

Diagnostic accuracy of sperm chromatin dispersion test to evaluate sperm deoxyribonucleic acid damage in men with unexplained infertility Cinthia M.

Wen-Ling Lee, M. D. , Wei-Min Liu, M. D. , Jong-Ling Fuh, M. D

Akanksha Mehta, M. D. , Alexander Bolyakov, M. S. , Peter N

Martin D. Keltz, M. D. , Josh C. Skorupski, M. D. , Katrina Bradley, M

Measurement of sperm DNA fragmentation using bright-field microscopy: comparison between sperm chromatin dispersion test and terminal uridine nick-end.

Influence of acupuncture stimulation on pregnancy rates for women undergoing embryo transfer Caroline Smith, Ph.D., Meaghan Coyle, B.Hlth.Sc. (Acup.),

Association between prior appendectomy and/or tonsillectomy in women and subsequent pregnancy rate: a cohort study Li Wei, Ph.D., Thomas MacDonald, M.D.,

DNA fragmentation of normal spermatozoa negatively impacts embryo quality and intracytoplasmic sperm injection outcome Conrado Avendaño, M.S., Anahí.

Application of three-dimensional fluorescence in situ hybridization to human preimplantation genetic diagnosis Li-Ying Yan, Ph.D., Jie Qiao, M.D., Yuan.

Bolan Yu, Ph. D. , Yanbin Qi, B. S. , Dan Liu, B. S

Management of tubal ectopic pregnancy: methotrexate and salpingostomy are preferred to preserve fertility Stephanie Beall, M.D., Ph.D., Alan H. DeCherney,

Severe intra-abdominal bleeding from neglected omental implantation of ectopic tissue after methotrexate treatment for persistent ectopic pregnancy Mei-Chu.

What is the best frame rate for evaluation of sperm motility in different species by computer-assisted sperm analysis? Cesare Castellini, Ph.D., Alessandro.

Akanksha Mehta, M.D., Darius A. Paduch, M.D., Ph.D.

Cheng-Yu Long, M. D. , Chun-Shuo Hsu, M. D. , Pei-Lin Shao, M. S

Evaluation of diagnostic testis biopsy and the repetition of testicular sperm extraction surgeries in infertility patients Alayman Hussein, M.B.B.C.H.,

Fragmentation of DNA in morphologically normal human spermatozoa

Poor sperm quality affects clinical outcomes of intracytoplasmic sperm injection in fresh and subsequent frozen–thawed cycles: potential paternal effects.

“Information-rich” reproductive outcomes in carriers of a structural chromosome rearrangement ascertained on the basis of recurrent pregnancy loss Michelle.

Lysed cell removal promotes frozen–thawed embryo development

Human papillomavirus found in sperm head of young adult males affects the progressive motility Carlo Foresta, M.D., Andrea Garolla, M.D., Daniela Zuccarello,

Cameron Gilbert, M. Sc. , Maria Valois, M. D. , FRCPC, Ph. D

Comparison of spindle and chromosome configuration in in vitro- and in vivo-matured mouse oocytes after vitrification Jack Y.J. Huang, M.D., Hai Ying.

Pregnancy outcome in infertile patients with polycystic ovary syndrome who were treated with metformin Samuel S. Thatcher, M.D., Ph.D., Elizabeth M.

Natalia Rougier, M. D. , Heydy Uriondo, M. Sc. , Sergio Papier, M. D

Reducing the dose of human chorionic gonadotropin in high responders does not affect the outcomes of in vitro fertilization David W. Schmidt, M.D.,,

Redefining the relationship between sperm deoxyribonucleic acid fragmentation as measured by the sperm chromatin structure assay and outcomes of assisted.

Fertility and Sterility: an evaluation

Comparison of mitochondrial DNA contents in human embryos with good or poor morphology at the 8-cell stage David Pei-Cheng Lin, Ph.D., Chun-Chia Huang,

Insulin growth factors may be implicated in human sperm capacitation

Seul Ki Kim, M. D. , Byung Chul Jee, M. D. , Ph. D. , Seok Hyun Kim, M

Yen-Ni Teng, Ph. D. , Yung-Ming Lin, M. D. , Hsiao-Fang Sunny Sun, Ph

A review and meta-analysis of prospective trials comparing different catheters used for embryo transfer William M. Buckett, M.D. Fertility and Sterility

David H. Barad, M.D., M.S., Norbert Gleicher, M.D.

Nerve fibers and menstrual cycle in peritoneal endometriosis

In vitro sildenafil citrate use as a sperm motility stimulant

Are sperm DNA fragmentation, hyperactivation, and hyaluronan-binding ability predictive for fertilization and embryo development in in vitro fertilization.

Contact among families who share the same sperm donor

P-24 Fertility and Sterility Volume 85, Pages S19-S20 (April 2006)

Is chromosome testing of the second miscarriage cost saving

Yinghui Ye, M. D. , Ph. D. , Chenming Xu, PhD. , Yuli Qian, B. Sc

Maternal serum screening markers and nuchal translucency measurements in in vitro fertilization pregnancies: a systematic review Andrea Lanes, M.Sc.,

Presentation transcript:

Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates Chun-Chia Huang, M.Sc., David Pei-Cheng Lin, Ph.D., Hui-Mei Tsao, M.Sc., Tzu-Chun Cheng, Ph.D., Chung-Hsien Liu, M.D., Ph.D., Maw-Sheng Lee, M.D., Ph.D. Fertility and Sterility Volume 84, Issue 1, Pages 130-140 (July 2005) DOI: 10.1016/j.fertnstert.2004.08.042 Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 An example of TUNEL assay results. The green cells in B (arrows) were regarded as positive, compared with the DAPI-stained blue cells in A on the same observation field. The cells with relatively weak green fluorescence were regarded as negative. Original magnification, ×600. Huang. Sperm DNA fragmentation reduces velocity. Fertil Steril 2005. Fertility and Sterility 2005 84, 130-140DOI: (10.1016/j.fertnstert.2004.08.042) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Raw scattered plots showing the fertilization rates and good embryo rates in correlation with sperm DNA fragmentation rates in the IVF/ICSI groups. Huang. Sperm DNA fragmentation reduces velocity. Fertil Steril 2005. Fertility and Sterility 2005 84, 130-140DOI: (10.1016/j.fertnstert.2004.08.042) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions