Analysis of the first mitotic spindles in human in vitro fertilized tripronuclear zygotes after pronuclear removal  Yi-Fan Gu, Ge Lin, Chang-Fu Lu, Guang-Xiu.

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Analysis of the first mitotic spindles in human in vitro fertilized tripronuclear zygotes after pronuclear removal  Yi-Fan Gu, Ge Lin, Chang-Fu Lu, Guang-Xiu Lu  Reproductive BioMedicine Online  Volume 19, Issue 5, Pages 745-754 (November 2009) DOI: 10.1016/j.rbmo.2009.09.013 Copyright © 2009 Reproductive Healthcare Ltd, Duck End Farm, Dry Drayton, Cambridge CB23 8DB, UK Terms and Conditions

Figure 1 Pronuclear removal of human 3PN zygotes. (A) Human 3PN zygote. (B) Human 3PN zygote after pronuclear removal. 400× magnification. Reproductive BioMedicine Online 2009 19, 745-754DOI: (10.1016/j.rbmo.2009.09.013) Copyright © 2009 Reproductive Healthcare Ltd, Duck End Farm, Dry Drayton, Cambridge CB23 8DB, UK Terms and Conditions

Figure 2 Polscope analysis of the first mitotic spindles in (A and C) human 3PN zygotes and (B and D) 3PN zygotes with pronuclear removal. (A and B) Normal bipolar spindle; (C and D) tripolar spindle. 200× magnification. Reproductive BioMedicine Online 2009 19, 745-754DOI: (10.1016/j.rbmo.2009.09.013) Copyright © 2009 Reproductive Healthcare Ltd, Duck End Farm, Dry Drayton, Cambridge CB23 8DB, UK Terms and Conditions

Figure 3 Confocal laser scanning microscopy images of the microtubules polymerizing in human 3PN zygotes. The microtubules begin to polymerize around pronuclei in human 3PN zygotes to form a tripolar spindle. Two sperm tails are attached to two male pronuclei, which are far from the second polar body. Red=α-tubulin; blue=DNA. 600× magnification; scale bar=40μm. Reproductive BioMedicine Online 2009 19, 745-754DOI: (10.1016/j.rbmo.2009.09.013) Copyright © 2009 Reproductive Healthcare Ltd, Duck End Farm, Dry Drayton, Cambridge CB23 8DB, UK Terms and Conditions

Figure 4 Confocal laser scanning microscopy images of the first mitotic spindles in (A, C, and E) human 3PN zygotes and (B, D, and F) 3PN zygotes with pronuclear removal. (A and B) Normal bipolar spindle; (C and D) tripolar spindle; (E and F) multi-spindle. Red=α-tubulin; blue=DNA. 600× magnification; scale bar=40μm. Reproductive BioMedicine Online 2009 19, 745-754DOI: (10.1016/j.rbmo.2009.09.013) Copyright © 2009 Reproductive Healthcare Ltd, Duck End Farm, Dry Drayton, Cambridge CB23 8DB, UK Terms and Conditions

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