Roles of systemic versus tumour SK1 in the regulation of local tumour growth and lung colonization/metastasis.A.Effects of siRNA‐mediated SK1 knockdown.

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Roles of systemic versus tumour SK1 in the regulation of local tumour growth and lung colonization/metastasis.A.Effects of siRNA‐mediated SK1 knockdown on MB49‐derived allograft tumour growth in the flanks of WT versus SK1−/− mice compared to non‐targeting Scr siRNA‐transfected controls were measured for 35 days (n = 6/group). Roles of systemic versus tumour SK1 in the regulation of local tumour growth and lung colonization/metastasis.A.Effects of siRNA‐mediated SK1 knockdown on MB49‐derived allograft tumour growth in the flanks of WT versus SK1−/− mice compared to non‐targeting Scr siRNA‐transfected controls were measured for 35 days (n = 6/group). Data are represented as mean ± SD. Error bars represent standard deviations. p < 0.05 (*) was considered significant.B.Efficacy of SK1 knockdown in response to siRNAs in MB49‐derived allografts was measured by RT‐PCR after surgical removal of tumours from the flanks of WT and SK1−/− mice. Data are represented as mean ± SD. Error bars represent standard deviations. p < 0.05 (*) was considered significant.C,D.Effects of siRNA‐mediated knockdown of SK1 in MB49 cells on lung colonization/metastasis (C) and lung weight or metastatic lung tumour nodules (D, left and right panels, respectively) were measured after tail vein injections for 19–21 days (n = 8/group). Data are represented as mean ± SD. Error bars represent standard deviations. p < 0.05 (*) was considered significant.E.Effects of the genetic loss of systemic SK1 on overall survival of WT and SK1−/− mice after tail vein injections of MB49 cells were measured for 35 days (n = 8/group).F.Effects of the pharmacologic inhibition of systemic S1P signalling using Sphingomab on lung colonization/metastasis of MB49 cells were measured for 16 days after tail vein injections compared to controls (n = 8/group). Data are represented as mean ± SD. Error bars represent standard deviations. p < 0.05 (*) was considered significant. Suriyan Ponnusamy et al. EMBO Mol Med. 2012;4:761-775 © as stated in the article, figure or figure legend