Hydrodynamic Gene Delivery: Its Principles and Applications

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Hydrodynamic Gene Delivery: Its Principles and Applications Takeshi Suda, Dexi Liu Molecular Therapy Volume 15, Issue 12, Pages 2063-2069 (December 2007) DOI: 10.1038/sj.mt.6300314 Copyright © 2007 The American Society of Gene Therapy Terms and Conditions

Figure 1 Proposed mechanism for hydrodynamic gene delivery to hepatocytes. (a) Architecture of the liver showing fenestrated endothelium in the center, surrounding hepatocytes and other cellular structures. For simplicity, one of two basal surfaces is drawn in each hepatocyte. (b) Initial impact of hydrodynamic injection on the liver showing that the injected solution (bright green) is forced out of the endothelium and into impacted hepatocytes. (c) Physical expansion of the liver showing stretched endothelium and swollen hepatocytes due to entry of DNA solution into cell interior. (d) Architecture of the liver showing recovered endothelium and transfected hepatocytes. Molecular Therapy 2007 15, 2063-2069DOI: (10.1038/sj.mt.6300314) Copyright © 2007 The American Society of Gene Therapy Terms and Conditions