Bacterial Sepsis Increases Survival in Metastatic Melanoma: Chlamydophila Pneumoniae Induces Macrophage Polarization and Tumor Regression  Krisztina Buzás, Annamária Marton, Csaba Vizler, Edina Gyukity-Sebestyén, Mária Harmati, Katalin Nagy, Ágnes Zvara, Róbert L. Katona, Vilmos Tubak, Valéria Endrész, István B. Németh, Judit Oláh, László Vígh, Tamás Bíró, Lajos Kemény  Journal of Investigative Dermatology  Volume 136, Issue 4, Pages 862-865 (April 2016) DOI: 10.1016/j.jid.2015.12.032 Copyright © 2015 The Authors Terms and Conditions

Figure 1 Chlamydophila pneumoniae treatment results in melanoma metastasis regression and increases survival of animals as well as of CD11b+ and CD80+ immune cell infiltration of tumor tissues. (a, c) Number of lung metastases, (b, d) melanoma antigen MART, and (t) survival rate of mock or Chlamydophila pneumoniae (CP)-treated immunocompetent C57BL/6 and immunodeficient NSG mice. (e–h) Representative images and hematoxylin and eosin-stained histological sections of dissected lungs of (e) mock and (f) CP-treated immunocompetent mice, as well as of (g) mock and (h) CP-treated immunodeficient (NSG) animals. Note that subpleural metastasis formation is extensive in diameter but not in thickness in NSG mice (arrowheads). Bars = 100 μm. (e) Trophical necroses indicate high tumor burden. Inset: atypical tumor cells and regions of necrosis. (f) Circles and right inset: foci of regressive metastases. Left inset: areas of residual pneumonitis after CP treatment. (g, h) In both mock and CP-treated NSG mice, miliary metastases were developed subpleurally (arrowheads) and intraparenchymally (circles) without significant inflammatory reactions (insets: higher magnification of intraparenchymal metastases). (i–l) Immunohistochemistry of (i, j) CD11b (diaminobenzidine, brown) and (k, l) CD80 (Fast red, red) on lungs of (i, k) mock or (j, l) CP-treated C57BL/6 mice. Dashed lines indicate tumor border. (i–r) Arrows indicate infiltrating immune cells. Intratumoral number of (m) CD11b+ and (n) CD80+ cells determined as a ratio of 100 tumor cells in C57BL/6 mice. (s) CD80+ cells in NSG lungs counted by square millimeter. Data are given as mean ± standard deviation. Journal of Investigative Dermatology 2016 136, 862-865DOI: (10.1016/j.jid.2015.12.032) Copyright © 2015 The Authors Terms and Conditions