Do Now 12/4 12 /4 Enzyme Inhibitors notes 30 WOD: FRAY (fray) n. fight or scuffle; brawl The party turned into a FRAY when the bikers showed up. A FRAY occurred in the cafeteria when Kirk spilled his lunch on Jody.  When Mark was beaned by the pitcher, a FRAY ensued between the teams. Study for your Enzyme quiz! Concepts to know: lock and key hypothesis Induced fit hypothesis vocab: substrate, enzyme, active site, activation energy reaction curves effects of temperature and pH on enzyme controlled reactions 12 /4 Enzyme Inhibitors notes 30

Enzyme Inhibitors INB Pg 30

Inhibition Active site of enzyme fits perfectly to substrate However, it is possible for another molecule to bind to an enzymes active site if it is very similar in shape to the enzyme’s substrate This would inhibit the enzyme’s function Substrate Inhibitor Enzyme

Competitive inhibition A competitive inhibitor is any compound which closely resembles the chemical structure and molecular geometry of the substrate. The inhibitor competes for the same active site as the substrate molecule.

Competitive inhibitors If a competitive inhibitor binds only briefly to the active site, there is competition between it and the substrate for the site Which one “wins” depends on which has a higher concentration http://www.youtube.com/watch?v=duN73LFWNlo

Competitive inhibition Usually reversible, because the inhibitor does not permanently bind to the enzyme Inhibition can be reversed by increasing concentration of substrate

Competitive inhibition example Methanol poisoning occurs because methanol is oxidized to formaldehyde and formic acid which attack the optic nerve causing blindness. Ethanol is given as an antidote for methanol poisoning because ethanol competitively inhibits the oxidation of methanol. Ethanol is oxidized in preference to methanol and consequently, the oxidation of methanol is slowed down so that the toxic by-products do not have a chance to accumulate.

Non-competitive inhibition Inhibition does not depend on substrate concentration Inhibitor will block enzyme function at low and high concentration of substrate Two main types Irreversible inhibition Allosteric inhibition

Non-competitive irreversible inhibition Sometimes, inhibitor can remain permanently bonded with the active site and therefore will cause an irreversible block to the substrate No competition occurs because no matter how much substrate is present, the active sites will be permanently occupied by the inhibitor http://www.youtube.com/watch?v=PILzvT3spCQ

Non-competitive irreversible inhibition Penicillin works by permanently occupying the active site of an enzyme that is essential for the synthesis of bacterial cell walls Penicillin and other β-lactam antibiotics act by inhibiting penicillin-binding proteins, which normally catalyze cross-linking of bacterial cell walls.

Non-competitive allosteric inhibition If a molecule can bind to another site on the enzyme (besides active site) and stop enzyme function, it is an allosteric inhibitor Can disrupt the 3D shape of enzyme molecule so active site cannot accept substrate Can be reversible or irreversible

End-product inhibition As an enzyme converts substrate to product, it is slowed down because the end product binds to another part of the enzyme and slows down its function Called negative feedback inhibition The more product = slower reaction Controls metabolic processes to stop enzyme from “running wild”

End product inhibition S P E E S E E I I

Homework project – 10 pts Using a sheet of computer paper and provided construction paper, design a poster that demonstrates: Competitive inhibiton Irreversible non competitive inhibition Allosteric inhibition End-product (negative feedback) inhibition Be sure to label the substrate, enzyme, and inhibitor! On the back of each picture, briefly describe (1-2 sent.) the mechanism of inhibition Due Friday @ end of class (will have time to work with partners in beginning)