Chapter 8 last part of lecture
DNA Microarrays monitor the expression of thousands of genes in one experiment MOVIE
Cluster Analysis used to identify sets of genes that are coordinately regulated The expression of 8600 genes (Columns) were analyzed under 12 time points. Red represents increase in expression green a decrease relative to untreated cells.
Cells can be genetically engineered to carry different types of mutations
Embryonic Stem (ES) cells can be genetically engineered and used to make a new animal
The genetically engineered ES cells are used to generate a chimeric animal, which is then used to make completely ES-derived animals
Chapter 15 Cell Communication
Signaling Cascade
Receptors can be on the cell surface or within the cell
Types of extracellular signaling
Types of extracellular signaling cont’d.
Endocrine signaling
Synaptic Signaling
Autocrine signaling
Signaling through channels found within the membrane MOVIE
Cells receive multiple stimuli that produce different responses MOVIE
The same ligand can have different effects depending upon the target cell line
Steroid hormones are lipophilic and interact with receptors found within the cell
Steroid receptors are transcription factors that are activated by binding to their ligand
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Signaling cascade serves to amplify the extracellular signal
Signaling Pathways from different Receptors overlap “cross-talk”