S. Manna, B. D. Ortika, E. M. Dunne, K. E. Holt, M. Kama, F. M

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A novel genetic variant of Streptococcus pneumoniae serotype 11A discovered in Fiji S. Manna, B.D. Ortika, E.M. Dunne, K.E. Holt, M. Kama, F.M. Russell, J. Hinds, C. Satzke Clinical Microbiology and Infection Volume 24, Issue 4, Pages 428.e1-428.e7 (April 2018) DOI: 10.1016/j.cmi.2017.06.031 Copyright © 2017 The Author(s) Terms and Conditions

Fig. 1 Phylogenetic analysis of serogroup 11 capsular polysaccharide genes from 11F-like isolates (PMP1342 and PMP1343) with serogroup 11 strains MNZ272 (11A), 8087/40 (11B), Eddy no. 53 (11C), 70/86 (11D), MNZ264 (11E) and 34356 (11F), (GenBank accession nos. GU074952, CR931654, CR931655, CR931656, GU074953, CR931657 respectively). Genes specific to serogroup 11 include; wchA (A), wchJ (B), wchK (C), wcyK (D), wcwC (E), wcrL (F), wzy (G), wcwT (H), wcwU (I), wzx (J), gct (K) and wcjE (L). Trees for wcwC and wcjE do not include sequences from 11B and 11C as these serotypes lack these genes. Using MEGA 6 package [21], DNA sequences were aligned using MUSCLE; and maximum likelihood of phylogeny trees were generated based on Tamura-Nei model. Scale bars represent number of substitutions per site. Statistical support for branches was determined by bootstrapping (1000 replicates) and are displayed as percentages. Clinical Microbiology and Infection 2018 24, 428.e1-428.e7DOI: (10.1016/j.cmi.2017.06.031) Copyright © 2017 The Author(s) Terms and Conditions

Fig. 2 Alignment of 11F-like WcrL amino acid sequence with 11A and 11F sequences generated by Clustal Omega. Identical residues are indicated with an asterisk; conserved residents, a colon; and semiconserved residues by a period respectively. Highlighted is residue 112 (A, alanine; N, asparagine). Clinical Microbiology and Infection 2018 24, 428.e1-428.e7DOI: (10.1016/j.cmi.2017.06.031) Copyright © 2017 The Author(s) Terms and Conditions

Fig. 3 Alignment of 11F-like gct DNA sequences with 11A and 11F sequences generated using Clustal Omega. Identical residues are indicated with an asterisk. Box represents homopolymeric region with mutation site. Clinical Microbiology and Infection 2018 24, 428.e1-428.e7DOI: (10.1016/j.cmi.2017.06.031) Copyright © 2017 The Author(s) Terms and Conditions

Fig. 4 Representative latex agglutination reactions of Statens Serum Institut (SSI) 11F and 11A reference strains, and an 11F-like isolate from this study. Latex reagents were prepared using SSI antisera (11b, 11c, 11f, 11g) as Quellung as previously described [22]. Clinical Microbiology and Infection 2018 24, 428.e1-428.e7DOI: (10.1016/j.cmi.2017.06.031) Copyright © 2017 The Author(s) Terms and Conditions