Coral Bleaching Experiment Aiptasia Zoox Density after a Cold Shock. Group 4 Moh Will Cal Merleen Jessica

Aiptasia and Cold Shock After a cold shock, Zoox density will decrease after re-warmed to ambient temperatures. The lower the temperature during the cold shocks, the lower the Zoox density after re-warmth to ambient temp. In conducting the cold shocks, a temperature will be reached where no additional Zoox loss will occur.

Methods We labeled four independent tubes and placed Aiptasia and water within each of those tubes and then placed the tubes in a closed container containing ice with a thermometer simultaneously checking the temperature. We measured the grams of Aiptasia within each tube. Every fifteen minutes, beginning at time equals zero, we removed a tube, placed the tube outside until it went back up to ambient temperature, and then measured Zoox density.

Cold Shock Experiment Tube # Weight (g) Time (min) Action Cup Temp (˚C) 1(control) 0.0702 00 Insert 2,3,4; process 1 25 2 .1109 15 take out 2 5 3 0.1014 30 take out 3 4 0.0915 45 take out 4

Zoox Density of Tubes (1-4) Tube #1 113.9 x 10^6 Zoox/g wet weight. Tube #2 33.8x10^6 Zoox/g wet weight Tube #3 34.5 x 10^6 Zoox/g wet weight Tube #4 32.7 x 10^6 Zoox/g wet weight

An error..... how? I’m perfect! As demonstrated by our data, it appears that their was a big gap in Zoox density from the first tube to the others. The similar measurements in Zoox density in tubes 2-4 indicate to us that once a certain temperature is achieved (around 2 degrees Celsius) no additional Zoox loss will occur.

Finally……. We will continue to replicate our experiment and hopefully we’ll get a constant rate of Zoox loss along with a constant temperature level in which no additional Zoox loss will occur.