Supplemental data figure 1.

Slides:

Advertisements

Similar presentations

Supplemental Figure 1 A No. at risk T T T

Advertisements

P449. p450 Figure 15-1 p451 Figure 15-2 p453 Figure 15-2a p453.

Tools for Molecular Biology Amplification. The PCR reaction is a way to quickly drive the exponential amplification of a small piece of DNA. PCR is a.

The polymerase chain reaction (PCR) rapidly

Real Time PCR = Quantitative PCR.

Supplementary file 3 Concentration of the plasmid containing a single copy of MusaSAP1 5’ UTR : 50 ng/μl Total length of the plasmid containing a single.

Qai Gordon and Maddy Marchetti. What is Polymerase Chain Reaction? Polymerase Chain Reaction ( PCR ) is a process that amplifies small pieces of DNA to.

The first step to model DTG-PCR Ji Youn Lee Cell and microbial engineering laboratory Seoul National University.

Polymerase Chain Reaction (PCR): DNA Replication in vitro DNA replication in vivo DNA replication in vivo –Occurs in S-phase –Helicase, primers, DNA polymerase.

QUANTITATIVE ANALYSIS OF AFRICAN SWINE FEVER VIRUS BY DIGITAL PCR

Result Introduction Methods

PCR conditions: 94ºC for 15 min

Hepatitis C Virus RNA Real-Time Quantitative RT-PCR Method Based on a New Primer Design Strategy Lida Chen, Wenli Li, Kuo Zhang, Rui Zhang, Tian Lu,

Clinical Validation of a New Triplex Real-Time Polymerase Chain Reaction Assay for the Detection and Discrimination of Herpes simplex Virus Types 1 and.

Supplemental Figure 1 A) B) C)

Principles of Real-Time Quantitative PCR Techniques

Supplemental Figure 2 A B vehicle

Figure 4. Effect of primer coating density on the SBE reaction

Direct DNA Amplification from Crude Clinical Samples Using a PCR Enhancer Cocktail and Novel Mutants of Taq Zhian Zhang, Milko B. Kermekchiev, Wayne.

John P. Jakupciak, Wendy Wang, Peter E

Hepatitis C Virus RNA Real-Time Quantitative RT-PCR Method Based on a New Primer Design Strategy Lida Chen, Wenli Li, Kuo Zhang, Rui Zhang, Tian Lu,

Pre-Clinical Validation of a Novel, Highly Sensitive Assay to Detect PML-RARα mRNA Using Real-Time Reverse-Transcription Polymerase Chain Reaction James.

Mutant Enrichment with 3′-Modified Oligonucleotides

Xuan Li, Carrie M. Stith, Peter M. Burgers, Wolf-Dietrich Heyer

One-Step Ligation on RNA Amplification for the Detection of Point Mutations Lei Zhang, Jingjing Wang, Mia Coetzer, Stephanie Angione, Rami Kantor, Anubhav.

Supplemental Figure 5 A B

Locked Nucleic Acids Can Enhance the Analytical Performance of Quantitative Methylation-Specific Polymerase Chain Reaction Karen S. Gustafson The Journal.

Direct DNA Amplification from Crude Clinical Samples Using a PCR Enhancer Cocktail and Novel Mutants of Taq Zhian Zhang, Milko B. Kermekchiev, Wayne.

Rapid Detection of the Epidermal Growth Factor Receptor Mutation in Non-Small-Cell Lung Cancer for Analysis of Acquired Resistance Using Molecular Beacons

Dual detection of Legionella pneumophila and Legionella species by real-time PCR targeting the 23S-5S rRNA gene spacer region G. Yang, R. Benson, T.

Gene quantification using real-time quantitative PCR

Optimization of a Relative Telomere Length Assay by Monochromatic Multiplex Real- Time Quantitative PCR on the LightCycler 480 Anthony Y.Y. Hsieh, Sara.

PCR -PCR replicates (or amplifies) the DNA many times so that a large enough sample can be analyzed.

Figure S1A. Supplementary Figure S1A. In vitro infectivity of vaccinia virus after exposure to cavitation. A dose of 1x106 vaccinia virus was mixed with.

Detection of HIV-1 Minority Variants Containing the K103N Drug-Resistance Mutation Using a Simple Method to Amplify RNA Targets (SMART) Kenneth Morabito,

MethySYBR, a Novel Quantitative PCR Assay for the Dual Analysis of DNA Methylation and CpG Methylation Density Pang-Kuo Lo, Hanano Watanabe, Pi-Chun.

Molecular diagnosis of viral hepatitis

Clinical Validation of a New Triplex Real-Time Polymerase Chain Reaction Assay for the Detection and Discrimination of Herpes simplex Virus Types 1 and.

Molecular Analysis of Circulating Cell-Free DNA from Lung Cancer Patients in Routine Laboratory Practice Stephan Bartels, Sascha Persing, Britta Hasemeier,

Volume 19, Issue 4, Pages (August 2005)

Diagnostic Testing for Pandemic Influenza in Singapore

Limitations and Practical Procedure in BclII-Ig Heavy Chain Gene Rearrangement Real- Time Quantitative Polymerase Chain Reaction Barbara Dessars, Pierre.

Evaluation of the Cepheid GeneXpert BCR-ABL Assay

Consensus strategy to quantitate malignant cells in myeloma patients is validated in a multicenter study by Peter Willems, Onno Verhagen, Christine Segeren,

Assessing Heteroplasmic Load in Leber's Hereditary Optic Neuropathy Mutation 3460G→A/MT-ND1 with A Real-Time PCR Quantitative Approach Anna Genasetti,

Multiplexed Detection of Anthrax-Related Toxin Genes

Use of Single Nucleotide Polymorphisms (SNP) and Real-Time Polymerase Chain Reaction for Bone Marrow Engraftment Analysis Dwight H. Oliver, Richard E.

Parametric and Polar Curves

International Standards and Reference Materials for Quantitative Molecular Infectious Disease Testing Roberta M. Madej, Jack Davis, Marcia J. Holden,

Heteroduplex Formation in SMN Gene Dosage Analysis

Olivier Gruselle, Thierry Coche, Jamila Louahed

The absence of ADCC by nivolumab in vitro.

Volume 14, Issue 19, Pages (October 2004)

Absence of the Caveolin-1 P132L Mutation in Cancers of the Breast and Other Organs Shinya Koike, Yasuhiro Kodera, Akimasa Nakao, Hiroji Iwata, Yasushi.

Real-time PCR in the microbiology laboratory

Autologous chondrocyte implantation (ACI) for aged patients: development of the proper cell expansion conditions for possible therapeutic applications

Consensus JH Gene Probes with Conjugated 3′-Minor Groove Binder for Monitoring Minimal Residual Disease in Acute Lymphoblastic Leukemia Michihiro Uchiyama,

High-Throughput Identification and Quantification of Candida Species Using High Resolution Derivative Melt Analysis of Panfungal Amplicons Tasneem Mandviwala,

Development of an Allele-Specific Real-Time PCR Assay for Discrimination and Quantification of p63 R279H Mutation in EEC Syndrome Vanessa Barbaro, Letizia.

Comparison of three methods to quantify urinary aquaporin-2 protein

Evaluation of the Abbott Investigational Use Only RealTime HIV-1 Assay and Comparison to the Roche Amplicor HIV-1 Monitor Test, Version 1.5 Michael T.

Volume 6, Issue 1, Pages (July 2002)

Optimized Allele-Specific Real-Time PCR Assays for the Detection of Common Mutations in KRAS and BRAF Alois H. Lang, Heinz Drexel, Simone Geller-Rhomberg,

Volume 27, Issue 5, Pages (September 2007)

Visual Format for Detection of Mycobacterium tuberculosis and M

Validation of ddPCR assay for detection of KrasLox-G12D allele using LNA-2 primers. Validation of ddPCR assay for detection of KrasLox-G12D allele using.

HPV Genotype Detection Using Hybrid Capture Sample Preparation Combined with Whole Genome Amplification and Multiplex Detection with Luminex XMAP Brian.

Principles of Quantitative PCR

Quantification of bcl-2/JH Fusion Sequences and a Control Gene by Multiplex Real- Time PCR Coupled with Automated Amplicon Sizing by Capillary Electrophoresis

Xiaorong Wang, Peter Baumann Molecular Cell

Presentation transcript:

Supplemental data figure 1. Assisting primer annealing by an anchor probe improves FLAG assay performance Serial dilutions of target DNA were analyzed with the FLAG assay in presence (open diamonds) or absence (solid diamonds) of the anchor oligonucleotide. Each sample was tested in at least 6 replicates in 2 different runs. Mean threshold cycles (mean Ct) and standard deviations (SD) obtained are reported in the table. The equations shown refer to the correlation curves that interpolate the data points in presence or absence of the anchor oligonucleotide.