Rotating Vessel Wall For the Adhesion of Cardiac Cells Roli Kumar BME Oral Presentation#1 Advisor: Dr. Marc Lin
Background Many surgical and radiational procedures have left areas of damaged tissue in the heart. Test new drugs study development and function of cardiac tissue
Objectives 3-D cardiac tissue from a monolayer of cardiac cells 3-D cell cultures contain specifications similar to in vivo: cell to cell and cell to matrix interactions composition of interstitial fluid Three dimensional Cultures of Prostatic Cells
Design Specifications of Rotating Vessel End over end mixing of the cells Continuous rotation of the vessel Max of .2 dyne/cm2 of shear stress Minimal fluid turbulence Bubble-free aeration eliminate hydrodynamic forces Solid Body Rotation Suspending the cells in a liquid medium.
Apparatus Set-Up Plastic Rod Rotating vessel Water Magnetic Stirrer
Work Completed Literature search was completed: Found a metal ball Cell disassociation Rotating-Wall Vessel for Prostate Cells Found a metal ball
Current Work I am meeting with Dr. Bader who does Cardiology research to learn how to disassociate and cardiac cell growth. Putting the apparatus together
Future Plan of Action Calculate the sheer stress applied on cells when rotated Literature search on a way to bubble-free aeration of cells while they are rotating