T Cell Receptor-Vβ Analysis Identifies a Dominant CD60+ CD26– CD49d– T Cell Clone in the Peripheral Blood of Sézary Syndrome Patients  Enrico Scala, Dr, Maria Grazia Narducci, Paolo Amerio, Giannandrea Baliva, Romeo Simoni, Lorena Silvestri, Pietro Puddu, Ornella De Pita, Giandomenico Russo  Journal of Investigative Dermatology  Volume 119, Issue 1, Pages 193-196 (July 2002) DOI: 10.1046/j.1523-1747.2002.18194.x Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Identification of the dominant TCR-Vβ12 from patient 5. (A) Multiparameter flow cytometry on peripheral blood lymphocytes showing that nearly all CD4+ T cells are TCR-Vβ12+. (B) CDR3 size profile of TCR-Vβ12 transcripts from healthy donor (left) showing Gaussian distribution of eight band, and from patient 5 (right) showing only one dominant peak. (C) Reverse transcription–PCR with specific primers for TCR-Vβ12. The quality of the cDNA sample was determined by amplification of the housekeeping GAPDH gene. (D) Sequencing of the CDR3 region of the TCR-Vβ amplified from RNA compared with the GenBank database. Journal of Investigative Dermatology 2002 119, 193-196DOI: (10.1046/j.1523-1747.2002.18194.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 TCR-Vβ analysis at T0 and after 6 mo. (A) The percentages of TCR-Vβ expansion within CD4+ T cells at T0 and after 6 mo are shown. In two cases, patient 6 (B,C) and patient 8 (D,E), a marked reduction in circulating TCR-Vβ reactive cells was found. Journal of Investigative Dermatology 2002 119, 193-196DOI: (10.1046/j.1523-1747.2002.18194.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions