CSCI2950-C Lecture 3 September 13, 2007.

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Presentation transcript:

CSCI2950-C Lecture 3 September 13, 2007

Comparative Genomic Hybridization (CGH) Measuring Mutations in Cancer Comparative Genomic Hybridization (CGH)

CGH Analysis (1) Divide genome into segments of equal copy number -0.5 0.5 Log2(R/G) Genomic position Copy number of adjacent probes correlated -0.5 0.5 Deletion Amplification Genomic position

CGH Analysis (2) Identify aberrations common to multiple samples Chromosome 3 of 26 lung tumor samples on mid-density cDNA array. Common deletion located in 3p21 and common amplification – in 3q. Samples

Ben-Dor et al. Results

Intervals

Stacks and Footprints

Results (Diskin et al.) Frequence

Results (Diskin, et al.) Stacks