12/30/2018 Education Pays

What do you want to be when you grow up? 12/30/2018 What do you want to be when you grow up? Many possibilities to use your biological education. Biologist Life Scientist Doctor/Nurse BioEngineer Chemical Engineer YOUR DECISION HAS $$$ CONSEQUENCES!

12/30/2018 Job Growth Growth rate is ~35%/yr

Salary Information Median Salary for B.S. =$35k/yr 12/30/2018 Salary Information Median Salary for B.S. =$35k/yr Starting Salary ~$24/yr-$30k/yr

12/30/2018 Catalase The Enzyme

Using Enzymes in Industry 12/30/2018 Using Enzymes in Industry

Catalase 2 H2O2 ----> 2 H2O + O2(g) Liver Enzyme Reaction 12/30/2018 Catalase Liver Enzyme Reaction decomposition of peroxide 2 H2O2 ----> 2 H2O + O2(g) Catalase acts as a catalyst increases the rate of reaction

12/30/2018 Rate Plot

12/30/2018 Catalase Enzyme

12/30/2018 Catalase - Tetramer

12/30/2018 Catalase Active Site Heam Site

12/30/2018 Catalase Mechanism 2 H2O2 <----> 2 H2O + O2(g)

12/30/2018 Catalase Mechanism

k1 Fast!! k2 Slow k1 = Enzyme-Substrate Association 12/30/2018 k1 Fast!! k2 Slow k1 = Enzyme-Substrate Association k1 Runs at 4x the Speed Limit, Diffusion Limit k1 = 4 x 108 Liter/(mole sec) k2 = Product Kickout from Active Site Much Slower than the Speed Limit Speed limit 1010 1/sec k2 ~10 1/sec

12/30/2018 Rate Plot

12/30/2018 Hayes’ Data

12/30/2018 Catalase Mechanism

Constant Determination 12/30/2018 Constant Determination

12/30/2018 Hayes’ Data

Effect of Catalase Concentration 12/30/2018 Effect of Catalase Concentration

12/30/2018 Effect of Temperature

12/30/2018 Effect of pH

12/30/2018 To Do Biology, you need Chemistry Mathematics Engineering!