(A) Immunohistochemistry of rat brainstem neurones reacting with anti-Hu antibodies showing strong nuclear staining and weaker cytoplasmic staining (counterstained with H&E). (A) Immunohistochemistry of rat brainstem neurones reacting with anti-Hu antibodies showing strong nuclear staining and weaker cytoplasmic staining (counterstained with H&E). (B) A Western blotting of anti-Hu serum against recombinant Hu antigen demonstrating the typical ladder banding pattern (courtesy of E Amyes and Professor A Vincent, Institute of Molecular Medicine, Oxford). J H Rees J Neurol Neurosurg Psychiatry 2004;75:ii43-ii50 ©2004 by BMJ Publishing Group Ltd