Group Name MRSA2 Group member name Karin Sundberg Marc Nielsen

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Presentation transcript:

Group Name MRSA2 Group member name Karin Sundberg Marc Nielsen Ana Freitas Maria Nummela Jie Xu

Isolates Sample name Subtype Notes ERR070040-P12 ST2371 From Patient 12 ERR124435-P24 From Patient 24 ERR124434-P22 From Patient 22 ERR128716-HW From Health Care Worker - misses the ermC plasmid ERR070035-not-outbreak ST8 Not part of the outbreak

Result of species identification (KmerFinder) Strain Species acc. to Harris et al 2013 Predicted species Closest matching strain Template coverage ERR070040-P12 S. aureus HO 50960412 97.43 ERR124435-P24 97.47 ERR124434-P22 97.36 ERR128716-HW ERR070035-not-outbreak USA_300_FPR3757 96.22

Representative KmerFinder result

Result of Multilocus Sequence Typing (MLST) Strain ST acc. to Harris et al 2014 Predicted ST ERR070040-P12 ST2371􀀃 ST-2371 ERR124435-P24 ERR124434-P22 ERR128716-HW ERR070035-not-outbreak ST8􀀃 ST-8

Representative Multilocus Sequence Typing result

Result of antibiotics resistance analysis Isolate Cefoxitin/Methicillin (MecA) Cipro-floxacin (chromo-somal mutation) Erythro-mycin (Erm(A), Erm(B), Erm(C)) Fusidic acid (chromo-somal mutation) Gentamicin (aac(6’)-aph(2'')) Mupirocin (chromo-somal mutation) Rifampicin (chromo-somal mutation) Tetra-cycline (tetC, tetM, tetR, tetS Vancomycin (Chromo-somal mutations or the vanA or vanB gene cluster.) Neomycin (aadD, aac(6')-aph(2'')) P12 RES RES (norA) RES (ermC) Unknown SUS P24 P22 RES (ermc) HW non-out-break RES (ermA) How is the agreement with the results presented in Harris et al 2013? Problems with “Unknown”, can’t make conclusions on resistance caused by chromosomal mutations.

Result of plasmid analysis Isolate rep10 repUS12 rep20 rep5 rep7 ERR070040-P12 2 27 13 ERR124435-P24 31 72 ERR124434-P22 10 25 65 ERR128716-HW 40 52 ERR070035-not-outbreak 3 For each strain write which node the replicon was found on. If not found, leave the cell empty. Which plasmid is the ermC gene on? Rep10 (pDLK1-like plasmid) The ermC gene is found on node 2 in P12, which also houses plasmid rep10. Similar for P22 (node 10).

Distance matrix made by NDtree and tree made with neighbour-joining

Distance matrix made by CSIPhylogeny and tree made with neighbour-joining