Exome Sequencing Identifies Mitochondrial Alanyl-tRNA Synthetase Mutations in Infantile Mitochondrial Cardiomyopathy Alexandra Götz, Henna Tyynismaa,

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Exome Sequencing Identifies Mitochondrial Alanyl-tRNA Synthetase Mutations in Infantile Mitochondrial Cardiomyopathy Alexandra Götz, Henna Tyynismaa, Liliya.

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Exome Sequencing Identifies Mitochondrial Alanyl-tRNA Synthetase Mutations in Infantile Mitochondrial Cardiomyopathy Alexandra Götz, Henna Tyynismaa, Liliya Euro, Pekka Ellonen, Tuulia Hyötyläinen, Tiina Ojala, Riikka H. Hämäläinen, Johanna Tommiska, Taneli Raivio, Matej Oresic, Riitta Karikoski, Outi Tammela, Kalle O.J. Simola, Anders Paetau, Tiina Tyni, Anu Suomalainen The American Journal of Human Genetics Volume 88, Issue 5, Pages 635-642 (May 2011) DOI: 10.1016/j.ajhg.2011.04.006 Copyright © 2011 The American Society of Human Genetics Terms and Conditions

Figure 1 The Mitochondrial Cardiomyopathy Patients and Their Families (A) Pedigrees of families 1 and 2. (B) Cytochrome c oxidase (COX) activity (shown in brown) of heart and skeletal muscle of patients 1 (II-1, family 1) and 2 (II-6, family 2). Simultaneous histochemical analysis for COX and succinate dehydrogenase (SDH, shown in blue) activities (Cox/Sdh) on frozen cryostat sections revealed mitochondrial COX deficiency with mitochondrial proliferation. (C) Blue native electrophoresis analyses of mitochondrial respiratory chain complexes in the heart, brain, and liver of patient 1 (P1), compared to control samples (c). Ten micrograms of sample protein was loaded onto a gel. For protein detection, monoclonal antibodies (Mitosciences) against the 39 kDa subunit of Complex I (CI), the subunits core 2 or Rieske of Complex III (CIII), the cox1p and cox4 subunits of Complex IV (CIV), and the 70 kDa-Ip subunit of Complex II (CII) were used. The American Journal of Human Genetics 2011 88, 635-642DOI: (10.1016/j.ajhg.2011.04.006) Copyright © 2011 The American Society of Human Genetics Terms and Conditions

Figure 2 Schematic Representation of the Exome Data Analysis and Data Filtering (1) The known dbSNP130 variants were excluded assuming the pathogenic variant to be too rare to (yet) exist in the database. (2) Nongenic variants were excluded. (3) Homozygous changes were selected: autosomal infantile-onset mitochondrial disorders are generally recessively inherited and homozygosity of the pathogenic variant was considered possible as the patient originated from Finland, a genetic isolate. (4) The consequence of the remaining variants was assessed by SIFT Genome tool (5) Genes encoding proteins with a mitochondrial function were predicted by MitoProt and TargetP. The American Journal of Human Genetics 2011 88, 635-642DOI: (10.1016/j.ajhg.2011.04.006) Copyright © 2011 The American Society of Human Genetics Terms and Conditions

Figure 3 AARS2 and Mutations (A) AARS2 mutation sequences in patients 1 (II-1, family 1) and 2 (II-6, family 2). (B) Cross-species protein conservation of mtAlaRS, flanking the altered amino acids p.Leu155Arg and p.Arg592Trp in mammals. The corresponding gene has not been fully characterized in other vertebrate species. (C) Schematic representation of AARS2. Boxes represent exons 1–22. Two main functional domains, the aminoacylation (pink) and editing (green) domains, are indicated. M denotes the mitochondrial targeting signal. The p.Leu155Arg mutation is located in the aminoacylation domain, p.Arg592Trp in the editing domain. (D) The AARS2-GFP fusion protein (green) colocalizes with Mitotracker Red (Invitrogen), indicating mitochondrial localization for mtAlaRS in HEK293T cells (overlay in yellow). The cells were examined and imaged with an Olympus IX8 fluorescence microscope. The American Journal of Human Genetics 2011 88, 635-642DOI: (10.1016/j.ajhg.2011.04.006) Copyright © 2011 The American Society of Human Genetics Terms and Conditions

Figure 4 Modeled Human mtAlaRS (A) The location of Leu155 within the aminoacylation domain (pink) and of Arg592 within the editing domain (green). The strand-loop-strand motif (aa 764–783) in the editing domain is shown in yellow. (B) Hydrophobic architectural residues surrounding the catalytic aminoacylation site. Carbon atoms of the catalytic residues involved in amino acid binding and aminoacyl adenylate formation are shown in magenta. (C) Conserved positively charged residues, predicted to be involved in the recognition and binding of tRNA (yellow) in the editing domain of human mtAlaRS. (D) Comparative analysis of the predicted secondary structures of human cytoplasmic and mitochondrial tRNAAla revealed that the identity base pair of the latter is likely to be G5:U64, implying a structurally unique tRNA recognition site in the human mtAlaRS. The American Journal of Human Genetics 2011 88, 635-642DOI: (10.1016/j.ajhg.2011.04.006) Copyright © 2011 The American Society of Human Genetics Terms and Conditions

Figure 5 Metabolomic Analysis: Postmortem Heart and Skeletal Muscle of Patient Shows Increased Alanine Levels Patient 1 (II-1, family 1) heart, muscle, and liver samples were compared to those of an age-matched patient with nonmitochondrial dilated CMP (heart and skeletal muscle) and those of a 10-year-old patient with mitochondrial encephalopathy (heart and liver). The American Journal of Human Genetics 2011 88, 635-642DOI: (10.1016/j.ajhg.2011.04.006) Copyright © 2011 The American Society of Human Genetics Terms and Conditions