Gemma D’Aniello, M. D. , Salvatore Ronsini, M. D. , Francesco Guida, M

Slides:



Advertisements
Similar presentations
What’s the delay? A qualitative study of women’s experiences of reaching a diagnosis of endometriosis  Karen Ballard, Ph.D., Karen Lowton, Ph.D., Jeremy.
Advertisements

Soyoung Bae, M.D., Joseph Karnitis, M.D.  Fertility and Sterility 
Who requests their sperm donor's identity
Local injury to the endometrium in controlled ovarian hyperstimulation cycles improves implantation rates  Liang Zhou, M.D., Rong Li, M.D., Ph.D., Rong.
Daily physical activity and hot flashes in the Study of Women's Health Across the Nation (SWAN) Flashes Study  Carolyn Gibson, M.P.H., M.S., Karen Matthews,
Akanksha Mehta, M.D., Mark Sigman, M.D.  Fertility and Sterility 
Fertility and Sterility  Volume 97, Issue 3, Pages (March 2012)
The relative effects of hormones and relationship factors on sexual function of women through the natural menopausal transition  Lorraine Dennerstein,
Fernando Zegers-Hochschild, M.D.  Fertility and Sterility 
Hormonal profile and endometrial morphology in letrozole-controlled ovarian hyperstimulation in ovulatory infertile patients  Armando Cortínez, M.D.,
Michael M. Alper, M.D.  Fertility and Sterility 
Inhibition of human sperm function and mouse fertilization in vitro by an antibody against cation channel of sperm 1: the contraceptive potential of its.
Brett McKinnon, Ph. D. , Nick A. Bersinger, Ph. D. , Alexander W
Clinical application of oocyte vitrification: a systematic review and meta-analysis of randomized controlled trials  Ana Cobo, Ph.D., César Diaz, M.D. 
Hepatitis and reproduction
The role of Chlamydia trachomatis infection in male infertility
The value of Chlamydia trachomatis–specific IgG antibody testing and hysterosalpingography for predicting tubal pathology and occurrence of pregnancy 
Antonino Forabosco, M.D., Ph.D., Chiarella Sforza, M.D., Ph.D. 
Early loss rates of entire pregnancies after assisted reproduction are lower in twin than in singleton pregnancies  Alexandra Matias, M.D., Giovanni B.
Can an educational DVD improve the acceptability of elective single embryo transfer? A randomized controlled study  Nicole Hope, M.B., B.S., Hon., Luk.
Satin S. Patel, M.D., Kelley S. Carrick, M.D., Bruce R. Carr, M.D. 
Medical treatment of ectopic pregnancy: a committee opinion
Double probing individual human spermatozoa: aniline blue staining for persistent histones and fluorescence in situ hybridization for aneuploidies  Laszlo.
Aberrant implantation and growth of uterine leiomyoma in the abdominal wall after laparoscopically assisted myomectomy  Osamu Wada-Hiraike, M.D., Ph.D.,
Impact of breast cancer chemotherapy on ovarian reserve: a prospective observational analysis by menstrual history and ovarian reserve markers  Andrea.
Ying-Hung Lin, Ph. D. , Chuan-Kai Chou, M. Sc. , Yu-Ching Hung, M. Sc
How old is too old? Challenges faced by clinicians concerning age cutoffs for patients undergoing in vitro fertilization  Robert L. Klitzman, M.D.  Fertility.
Oocyte cryopreservation
Curcumin ameliorates aflatoxin-induced toxicity in mice spermatozoa
Detection of oncogenic human papillomavirus genotypes on spermatozoa from male partners of infertile couples  Rosaria Schillaci, Ph.D., Giuseppina Capra,
Dana B. McQueen, M. D. , M. A. S. , Candice O. Perfetto, M. D
Mieke Carine Wim Eeckhaut, Ph.D.  Fertility and Sterility 
Looking back: egg donors' retrospective evaluations of their motivations, expectations, and experiences during their first donation cycle  Nancy J. Kenney,
A comparison of three downregulation approaches for poor responders undergoing in vitro fertilization  Laura Detti, M.D., Daniel B. Williams, M.D., Jared.
Management of tubal ectopic pregnancy: methotrexate and salpingostomy are preferred to preserve fertility  Stephanie Beall, M.D., Ph.D., Alan H. DeCherney,
Clomiphene citrate at 50: the dawning of assisted reproduction
Fertility status in male cystinosis patients treated with cysteamine
Letrozole stimulates the growth of human endometrial explants cultured in three- dimensional fibrin matrix  Mozafar Khazaei, Ph.D., Azadeh Montaseri, M.Sc.,
Introduction Fertility and Sterility
Akanksha Mehta, M.D., Darius A. Paduch, M.D., Ph.D. 
Michelle Rijsdijk, M.Med.Sc., Daniel Rossouw Franken, Ph.D. 
“Information-rich” reproductive outcomes in carriers of a structural chromosome rearrangement ascertained on the basis of recurrent pregnancy loss  Michelle.
A longitudinal study of the effects of free testosterone and other psychosocial variables on sexual function during the natural traverse of menopause 
Live babies born per oocyte retrieved in a subpopulation of oocyte donors with repetitive reproductive success  J. Ryan Martin, M.D., Jason G. Bromer,
Immunofluorescence Localization Of Epithelial Cadherin, Cross Reactive With An Antibody Against Human Placental Protein, In Human Preimplantation Embryos 
GnRH antagonists may affect endometrial receptivity
Cumulus cells and their extracellular matrix affect the quality of the spermatozoa penetrating the cumulus mass  Shun-Jia Hong, Ph.D., Philip Chi-Ngong.
One intrauterine device lost, two found
P-425 Fertility and Sterility Volume 86, Issue 3, (September 2006)
Cameron Gilbert, M. Sc. , Maria Valois, M. D. , FRCPC, Ph. D
Website quality assessment: Mistaking apples for oranges
Pregnancy outcome in infertile patients with polycystic ovary syndrome who were treated with metformin  Samuel S. Thatcher, M.D., Ph.D., Elizabeth M.
L. Scott, J. Bernsten, K. DeLegge, J. Hill, N. Ramsing 
Relaxin has a role in establishing a renal response in pregnancy
Fertility and Sterility: an evaluation
Insulin growth factors may be implicated in human sperm capacitation
Impact of subclinical hypothyroidism in women with recurrent early pregnancy loss  Lia A. Bernardi, M.D., Ronald N. Cohen, M.D., Mary D. Stephenson, M.D.,
Counteraction during movement of spermatozoa by Trichomonas vaginalis observed by visual image analysis: a possible cause of female infertility  Viroj.
Association of endometriosis with body size and figure
Current evaluation of amenorrhea
A simplified novel laparoscopic formation of neovagina for cases of Mayer-Rokitansky- Küster-Hauser syndrome  Atef Mohammad Darwish, M.D.  Fertility and.
Aline Ketefian, M. D. , Jianfang Hu, Ph. D. , Alfred A. Bartolucci, Ph
Nerve fibers and menstrual cycle in peritoneal endometriosis
In vitro sildenafil citrate use as a sperm motility stimulant
Soluble HLA-G in the peritoneal fluid of women with endometriosis
What’s the delay? A qualitative study of women’s experiences of reaching a diagnosis of endometriosis  Karen Ballard, Ph.D., Karen Lowton, Ph.D., Jeremy.
Contact among families who share the same sperm donor
Reda Mahfouz, M. D. , Rakesh Sharma, Ph. D. , Dipika Sharma, M. D
Are we underutilizing Palmer's point entry in gynecologic laparoscopy?
P-24 Fertility and Sterility Volume 85, Pages S19-S20 (April 2006)
Presentation transcript:

Occurrence of D-aspartic acid in human seminal plasma and spermatozoa: Possible role in reproduction  Gemma D’Aniello, M.D., Salvatore Ronsini, M.D., Francesco Guida, M.D., Patrizia Spinelli, Ph.D., Antimo D’Aniello, Ph.D.  Fertility and Sterility  Volume 84, Issue 5, Pages 1444-1449 (November 2005) DOI: 10.1016/j.fertnstert.2005.05.019 Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Immunohistochemical localization of D-Asp in human spermatozoa from normospermic donors. Human spermatozoa from normospermic donors were treated with (A) antibody against D-aspartate raised in rabbit and the peroxidase-DAB system, or (B) omission of the primary antibody. The positive staining reaction (A) occurs in all areas of the spermatozoa, indicating that D-Asp is present in both acrosome and nucleus. Magnification, ×1,000. D’Aniello. D-aspartic acid in human spermatozoa. Fertil Steril 2005. Fertility and Sterility 2005 84, 1444-1449DOI: (10.1016/j.fertnstert.2005.05.019) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Immuno-electron localization of D-Asp in human spermatozoa from normospermic donors. This is a typical example of human spermatozoa from normospermic donors, treated with (A) an antibody against D-aspartate as primary antibody, and as secondary antibody, the antirabbit IgG coupled to gold particles (in this case, the gold particles are well established as being localized in both acrosome and nucleus, demonstrating that D-Asp occurs in both acrosome and nucleus); or (B) omission of antibody against D-aspartate (control). Magnification, ×60,000. Scale bar = 0.66 μm. D’Aniello. D-aspartic acid in human spermatozoa. Fertil Steril 2005. Fertility and Sterility 2005 84, 1444-1449DOI: (10.1016/j.fertnstert.2005.05.019) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions