Development and Evaluation of a Pan-Sarcoma Fusion Gene Detection Assay Using the NanoString nCounter Platform Kenneth T.E. Chang, Angela Goytain, Tracy.

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Development and Evaluation of a Pan-Sarcoma Fusion Gene Detection Assay Using the NanoString nCounter Platform Kenneth T.E. Chang, Angela Goytain, Tracy Tucker, Aly Karsan, Cheng-Han Lee, Torsten O. Nielsen, Tony L. Ng The Journal of Molecular Diagnostics Volume 20, Issue 1, Pages 63-77 (January 2018) DOI: 10.1016/j.jmoldx.2017.09.007 Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Schematic of the probe design for fusion gene detection using the NanoString nCounter Elements platform. In the CodeSet, each fusion junction is represented by three oligonucleotide probes. Probe A binds the RNA transcript across the fusion junction. Probe B binds directly 3′ of probe A on the RNA transcript. A protector probe binds part of probe A when it is not bound to an RNA transcript for increased specificity. A reporter tag containing a unique optical barcode specific to the fusion break point is attached to each probe A for multiplexed analysis. A biotin tag is attached to each probe B for attachment to a streptavidin-coated cartridge. The Journal of Molecular Diagnostics 2018 20, 63-77DOI: (10.1016/j.jmoldx.2017.09.007) Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions