Partial knockdown of MPST markedly attenuates while overexpression of MPST exacerbates FFA-induced fat accumulation in hepatocyte in vitro. Partial knockdown.

Slides:

Advertisements

Similar presentations

Signal regulatory protein-α interacts with the insulin receptor contributing to muscle wasting in chronic kidney disease Sandhya S. Thomas, Yanjun Dong,

Advertisements

From: MicroRNA let-7 Regulates 3T3-L1 Adipogenesis

Volume 5, Issue 3, Pages (November 2013)

Platelet-Derived Growth Factor-BB Mediates Cell Migration through Induction of Activating Transcription Factor 4 and Tenascin-C Kristine P. Malabanan,

SiRNA Knockdown of Tissue Inhibitor of Metalloproteinase-1 in Keloid Fibroblasts Leads to Degradation of Collagen Type I Masayo Aoki, Koichi Miyake,

by Matilde Murga, Oscar Fernandez-Capetillo, and Giovanna Tosato

Juewon Kim, Hyunjung Choi, Eun-Gyung Cho, Tae R. Lee

Expression of adenylyl cyclases and miR‐142‐3p in CD4+CD25− T cells and CD4+CD25+ regulatory T cells. Expression of adenylyl cyclases and miR‐142‐3p in.

miR-133a positively regulated p53/p21 pathway.

PFKFB3, a Direct Target of p63, Is Required for Proliferation and Inhibits Differentiation in Epidermal Keratinocytes Robert B. Hamanaka, Gökhan M. Mutlu

Expression of GRIM-19 in missed abortion and possible pathogenesis

ORAI1 Ca2+ Channels Control Endothelin-1-Induced Mitogenesis and Melanogenesis in Primary Human Melanocytes Hedwig Stanisz, Alexandra Stark, Tatiana.

Signal regulatory protein-α interacts with the insulin receptor contributing to muscle wasting in chronic kidney disease Sandhya S. Thomas, Yanjun Dong,

FOXP3 regulates cAMP production by regulating miR‐142‐3p and AC9.

Volume 137, Issue 2, Pages e2 (August 2009)

Development of Cell-Penetrating Asymmetric Interfering RNA Targeting Connective Tissue Growth Factor Jihye Hwang, Chanil Chang, Ji Hyun Kim, Chang Taek.

Volume 138, Issue 3, Pages e2 (March 2010)

Intermittent cyclic mechanical tension promotes endplate cartilage degeneration via canonical Wnt signaling pathway and E-cadherin/β-catenin complex cross-talk

Volume 134, Issue 1, Pages (January 2008)

Identification of miR-145 as a Key Regulator of the Pigmentary Process

Silver Nanoparticle–Induced hMSC Proliferation Is Associated with HIF-1α-Mediated Upregulation of IL-8 Expression Sung Kyu Jung, Jin Hee Kim, Hee Joo.

Bone Marrow-Derived Mesenchymal Stem Cells Expressing Thioredoxin 1 Attenuate Bleomycin-Induced Skin Fibrosis and Oxidative Stress in Scleroderma Miao.

Expression of Protease-Activated Receptor-2 in SZ95 Sebocytes and its Role in Sebaceous Lipogenesis, Inflammation, and Innate Immunity Sang E. Lee, Ji-Min.

SREBP-1 pathway is involved in MPST-regulated fat accumulation in hepatocytes. SREBP-1 pathway is involved in MPST-regulated fat accumulation in hepatocytes.

Partial knockdown of MPST upregulates CSE by interacting with CSE

Partial knockdown of MPST markedly ameliorates hepatic steatosis in vivo. Partial knockdown of MPST markedly ameliorates hepatic steatosis in vivo. (A–C)

MPST attenuates H2S production and subsequently promotes fat accumulation in hepatocytes through CSE dependent-mechanism. MPST attenuates H2S production.

Lysophosphatidic Acid Promotes Cell Migration through STIM1- and Orai1-Mediated Ca2+i Mobilization and NFAT2 Activation Ralph Jans, Laura Mottram, Darren.

Sensitization of Melanoma Cells for Death Ligand TRAIL Is Based on Cell Cycle Arrest, ROS Production, and Activation of Proapoptotic Bcl-2 Proteins Sandra-Annika.

Volume 133, Issue 5, Pages (November 2007)

Ji-Young Kim, Tae-Ryong Lee, Ai-Young Lee

Volume 7, Issue 4, Pages (April 2008)

Substance P Stimulates Endothelin 1 Secretion via Endothelin-Converting Enzyme 1 and Promotes Melanogenesis in Human Melanocytes Phil June Park, Tae.

A Varp-Binding Protein, RACK1, Regulates Dendrite Outgrowth through Stabilization of Varp Protein in Mouse Melanocytes Soujiro Marubashi, Norihiko Ohbayashi,

Volume 84, Issue 2, Pages (August 2013)

The Eumelanin Intermediate 5,6-Dihydroxyindole-2-Carboxylic Acid Is a Messenger in the Cross-Talk among Epidermal Cells Daniela Kovacs, Enrica Flori,

Min Qin, Aslan Pirouz, Myung-Hwa Kim, Stephan R. Krutzik, Hermes J

Min Qin, Aslan Pirouz, Myung-Hwa Kim, Stephan R. Krutzik, Hermes J

Volume 5, Issue 3, Pages (November 2013)

Volume 5, Issue 5, Pages (November 2015)

Volume 19, Issue 7, Pages (May 2017)

The IL-6 Trans-Signaling-STAT3 Pathway Mediates ECM and Cellular Proliferation in Fibroblasts from Hypertrophic Scar Sutapa Ray, Xiaoxi Ju, Hong Sun,

YAP1 and IGF2BP3 regulate each other during fetal programming.

Effectively regenerating livers transiently accumulate proliferative IGF2BP3-positive cells. Effectively regenerating livers transiently accumulate proliferative.

Expression of Perilipin A on the Surface of Lipid Droplets Increases along with the Differentiation of Hamster Sebocytes In Vivo and In Vitro Noriko.

Knockdown of MPST weakens JNK phosphorylation, ameliorates hepatic oxidative stress and suppresses the release of MCP-1. Knockdown of MPST weakens JNK.

S100A1 and S100B mediate the suppression of terminal differentiation of chondrocytes by the SOX trio. S100A1 and S100B mediate the suppression of terminal.

USP2a overexpression modifies the microRNA expression profile of prostate cells. USP2a overexpression modifies the microRNA expression profile of prostate.

Sox4 promotes hepatic TG accumulation in vivo.

Volume 24, Issue 10, Pages (October 2016)

Impaired in vitro adipogenic differentiation parallels elevated HDAC9 expression. Impaired in vitro adipogenic differentiation parallels elevated HDAC9.

Fig. 6. RhoB is required for Rnd3-induced stress fibre formation

BCL-3 regulates expression of stemness-associated Wnt targets.

Fig. 6 Metarrestin treatment reduces pre-RNA synthesis and Pol I occupancy at rDNA without changing rDNA chromatin states. Metarrestin treatment reduces.

Fig. 4 Cdc42 enhances the cellular uptake of EVs.

Effect of sulindac and atorvastatin on the cell cycle.

Glial cell line-derived neurotrophic factor (GDNF) induces hepatic stellate cell (HSC) activation in human HSCs. Gdnf mRNA expression as determined by.

Cox regression analysis of the proportion of patients remaining in remission during azathioprine treatment related to minimum observed white blood cell.

Changes of gene expression induced by 1 mmol/L valproic acid in vitro.

Secretion of TNF-α from macrophages and monocytic cells were reduced by the deletion of Nrdc. Secretion of TNF-α from macrophages and monocytic cells were.

Fig. 2 Viperin promotes methionine oxidation of KSHV helicase.

The effects of HDAC2 knockdown on cell-cycle proteins.

Levels and activity of c-myc are increased in a series of stably transfected DAOY and UW228 medulloblastoma cell lines. Levels and activity of c-myc are.

Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization in 231BR3 cells. Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization.

DDX5 localizes to promoters of DNA replication genes and functions in the loading of RNA polymerase II onto these promoters. DDX5 localizes to promoters.

Administration of anti-glial cell line-derived neurotrophic factor (anti-GDNF) antibodies reduces liver fibrosis development in mice. Administration of.

Complete intestinal metaplasia (IM) is characterised by goblet cells stained red or blue by alcian blue (pH 2.5)-periodic acid Schiff stain and by the.

Glial cell line-derived neurotrophic factor (GDNF) is upregulated in mouse liver fibrosis. Glial cell line-derived neurotrophic factor (GDNF) is upregulated.

Glial cell line-derived neurotrophic factor (GDNF) induces hepatic stellate cell (HSC) activation via the activin receptor-like kinase 5 (ALK5)/Smad pathway.

Fig. 2 hTERT induces expression of heat shock protein genes through HSF1 and interacts with Hsp70-1. hTERT induces expression of heat shock protein genes.

Presentation transcript:

Partial knockdown of MPST markedly attenuates while overexpression of MPST exacerbates FFA-induced fat accumulation in hepatocyte in vitro. Partial knockdown of MPST markedly attenuates while overexpression of MPST exacerbates FFA-induced fat accumulation in hepatocyte in vitro. (A–C) After transfection with scrambled siRNA (negative control) and MPST siRNA for 24 hours, L02 cells were treated with FFAs for 24 hours. (A) Western blot and qPCR analyses were performed to determine MPST expression. (B) Representative images of Oil Red O staining of L02 cells is shown. Original magnification ×400. (C) Intracellular TG and TC contents were determined. (D–F) After transfecting with plasmid containing GFP with or without full-length MPST DNA for 24 hours, L02 cells were treated with FFAs for 24 hours. (D) Western blot and qPCR analyses were performed to determine MPST expression. (E) Representative images of Oil Red O staining of L02 cells is shown. Original magnification ×400. (F) The intracellular TG and TC contents were determined. The results are expressed as the mean±SD of three independent experiments. *p<0.05, **p<0.01, ***p<0.001. FFA, free fatty acid; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GFP, green fluorescent protein; MPST, 3-mercaptopyruvate sulfurtransferase; mRNA, messenger RNA; qPCR, quantitative PCR; siRNA, small interfering RNA; TC, total cholesterol; TG, triglyceride. Meng Li et al. Gut 2018;67:2169-2180 Copyright © BMJ Publishing Group Ltd & British Society of Gastroenterology. All rights reserved.