Characteristics of lymphocyte subsets in HIV-infected, long-term nonprogressor, and healthy Asian children through 12 years of age Jintanat Ananworanich,

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Characteristics of lymphocyte subsets in HIV-infected, long-term nonprogressor, and healthy Asian children through 12 years of age Jintanat Ananworanich, MD, PhD, Tanakorn Apornpong, MSc, Pope Kosalaraksa, MD, Tanyathip Jaimulwong, BSc, Rawiwan Hansudewechakul, MD, Chitsanu Pancharoen, MD, Torsak Bunupuradah, MD, Mom Chandara, MSc, Thanyawee Puthanakit, MD, Chaiwat Ngampiyasakul, MD, Jurai Wongsawat, MD, Suparat Kanjanavanit, MD, Wicharn Luesomboon, MD, Phennapha Klangsinsirikul, PhD, Nicole Ngo-Giang-Huong, PharmD, PhD, Stephen J. Kerr, PhD, Sasiwimol Ubolyam, MSc, Tawan Mengthaisong, RN, Rebecca S. Gelman, PhD, Kovit Pattanapanyasat, PhD, Vonthanak Saphonn, MD, PhD, Kiat Ruxrungtham, MD, MSc, William T. Shearer, MD, PhD Journal of Allergy and Clinical Immunology Volume 126, Issue 6, Pages 1294-1301.e10 (December 2010) DOI: 10.1016/j.jaci.2010.09.038 Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Comparison of cell subsets among 4 age groups of HIV-positive and healthy children. A, CD45+/CD3+/CD19− (total T cells). B, CD45+/CD3+/CD4+ (TH cells). C, CD45+/CD3+/CD8+ (cytotoxic T cells). D, CD45+/CD3−/CD19+ (B cells). E, CD45+/CD3−/CD16+/CD56+ (NK cells). ∗P < .05. No. of children: <2 to 3 years (n = 79), 4 to 5 years (n = 74), 6 to 8 years (n = 135), 9 to 12 years (n = 76). Pos, Positive. Journal of Allergy and Clinical Immunology 2010 126, 1294-1301.e10DOI: (10.1016/j.jaci.2010.09.038) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Comparison of cell subsets among 4 age groups of HIV-positive and healthy children. A, CD45+/CD3+/CD19− (total T cells). B, CD45+/CD3+/CD4+ (TH cells). C, CD45+/CD3+/CD8+ (cytotoxic T cells). D, CD45+/CD3−/CD19+ (B cells). E, CD45+/CD3−/CD16+/CD56+ (NK cells). ∗P < .05. No. of children: <2 to 3 years (n = 79), 4 to 5 years (n = 74), 6 to 8 years (n = 135), 9 to 12 years (n = 76). Pos, Positive. Journal of Allergy and Clinical Immunology 2010 126, 1294-1301.e10DOI: (10.1016/j.jaci.2010.09.038) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Comparison of cell subsets among 4 age groups of HIV-positive and healthy children. A, CD45+/CD3+/CD19− (total T cells). B, CD45+/CD3+/CD4+ (TH cells). C, CD45+/CD3+/CD8+ (cytotoxic T cells). D, CD45+/CD3−/CD19+ (B cells). E, CD45+/CD3−/CD16+/CD56+ (NK cells). ∗P < .05. No. of children: <2 to 3 years (n = 79), 4 to 5 years (n = 74), 6 to 8 years (n = 135), 9 to 12 years (n = 76). Pos, Positive. Journal of Allergy and Clinical Immunology 2010 126, 1294-1301.e10DOI: (10.1016/j.jaci.2010.09.038) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Comparison of cell subsets between HIV-positive and healthy children for 4 age groups. CD4+/45RA+/62L+ (naive TH cells). ∗P < .05. No. of children: <2 to 3 years (n = 79), 4 to 5 years (n = 74), 6 to 8 years (n = 135), 9 to 12 years (n = 76). Pos, Positive. Journal of Allergy and Clinical Immunology 2010 126, 1294-1301.e10DOI: (10.1016/j.jaci.2010.09.038) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Comparison of cell subsets between HIV-positive and healthy children for 4 age groups. CD3+/4+/45RO+ (memory TH cells). ∗P < .05. No. of children: <2 to 3 years (n = 79), 4 to 5 years (n = 74), 6 to 8 years (n = 135), 9 to 12 years (n = 76). Pos, Positive. Journal of Allergy and Clinical Immunology 2010 126, 1294-1301.e10DOI: (10.1016/j.jaci.2010.09.038) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Comparison of cell subsets between HIV-positive and healthy children for 4 age groups. CD4+/DR+/38+ (activated TH cells). ∗P < .05. No. of children: <2 to 3 years (n = 79), 4 to 5 years (n = 74), 6 to 8 years (n = 135), 9 to 12 years (n = 76). Pos, Positive. Journal of Allergy and Clinical Immunology 2010 126, 1294-1301.e10DOI: (10.1016/j.jaci.2010.09.038) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Comparison of cell subsets between HIV-positive and healthy children for 4 age groups. CD8+/45RA+/62L+ (naive cytotoxic T cells). ∗P < .05. No. of children: <2 to 3 years (n = 79), 4 to 5 years (n = 74), 6 to 8 years (n = 135), 9 to 12 years (n = 76). Pos, Positive. Journal of Allergy and Clinical Immunology 2010 126, 1294-1301.e10DOI: (10.1016/j.jaci.2010.09.038) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Comparison of cell subsets between HIV-positive and healthy children for 4 age groups. CD3+/4-/45RO+ (memory cytotoxic T cells). ∗P < .05. No. of children: <2 to 3 years (n = 79), 4 to 5 years (n = 74), 6 to 8 years (n = 135), 9 to 12 years (n = 76). Pos, Positive. Journal of Allergy and Clinical Immunology 2010 126, 1294-1301.e10DOI: (10.1016/j.jaci.2010.09.038) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Comparison of cell subsets between HIV-positive and healthy children for 4 age groups. CD8+/DR+/38+ (activated cytotoxic T cells). ∗P < .05. No. of children: <2 to 3 years (n = 79), 4 to 5 years (n = 74), 6 to 8 years (n = 135), 9 to 12 years (n = 76). Pos, Positive. Journal of Allergy and Clinical Immunology 2010 126, 1294-1301.e10DOI: (10.1016/j.jaci.2010.09.038) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions