DTT quenches the passive reference signal in real-time PCR

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Presentation transcript:

DTT quenches the passive reference signal in real-time PCR Lina Boiso, Malin Sanga, Johannes Hedman  Forensic Science International: Genetics Supplement Series  Volume 5, Pages e5-e6 (December 2015) DOI: 10.1016/j.fsigss.2015.09.003 Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Quenching of passive reference dye fluorescence by DTT. Fluorescence signals of the small autosomal fragment, IPC and passive reference are shown. (A) Extraction negative control without DTT, (B) Extraction negative control with DTT (∼4mM in the qPCR reaction). Forensic Science International: Genetics Supplement Series 2015 5, e5-e6DOI: (10.1016/j.fsigss.2015.09.003) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

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