Tobacco consumption and benzo(a)pyrene-diol-epoxide–DNA adducts in spermatozoa: in smokers, swim-up procedure selects spermatozoa with decreased DNA damage  Jeanne Perrin, M.D., Ph.D., Virginie Tassistro, M.Sc., Marion Mandon, M.Sc., Jean-Marie Grillo, M.D., Ph.D., Alain Botta, M.D., Ph.D., Pharm.D., Irene Sari-Minodier, M.D., Ph.D.  Fertility and Sterility  Volume 95, Issue 6, Pages 2013-2017 (May 2011) DOI: 10.1016/j.fertnstert.2011.02.021 Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Immunodetection of benzo(a)pyrene-diol-epoxide (BPDE)–DNA adducts in spermatozoa from nonsmoking (A–D) and smoking (E–H) patients. Panels A, B, E, and F show selected spermatozoa (SEL-SPZ), and C, D, G, and H show nonselected spermatozoa (NONSEL-SPZ). Panels K and L show human lymphocytes treated with 40 μmol/L BPDE (positive internal control samples); I and J show untreated human lymphocytes (negative internal control samples). Spermatozoa and lymphocytes were stained with Alexa 488 (green), and nuclei were counterstained with DAPI (blue). Original magnification ×1,000. Fertility and Sterility 2011 95, 2013-2017DOI: (10.1016/j.fertnstert.2011.02.021) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions